Báo cáo khoa học: "Comparison of cardiac function and coronary angiography between conventional pigs and micropigs as measured by multidetector row computed tomography" docx
Tài liệu Báo cáo khoa học: Attenuation of cardiac mitochondrial dysfunction by melatonin in septic mice ppt
... lLof50mm potassium phosphate buffercontaining 1 mm EDTA-K2(pH 7.4), and the oxidation of NADPH was spectrophotometrically measured for 3 min at340 nm [74]. The activity of GPx and GRd was ... iNOS. Ten nanograms of RNA extracted from mouse heartwas used, and quantification of iNOS mRNA was performed by real-time RT-PCR. The relative level was calculated as the ratio of inflammatory mRNA ... induction of inducible nitric oxide synthase duringsepsis was related to the increase of inducible mitochondrial nitric oxide syn-thase activity, as both inducible nitric oxide synthase and inducible...
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Báo cáo khoa học: Dissection of LolB function – lipoprotein binding, membrane targeting and incorporation of lipoproteins into lipid bilayers potx
... system, as described in (B). The densi-ties of the bands were determined and plotted as a function of theamounts of LolB and mLolB. LolB and mLolB (each 1 lg) wereanalysed by SDS–PAGE, followed by ... liposomes and mLolB. In contrast, Pal remainedsoluble when either mLolB or liposomes were omitted.The amount of Pal incorporated into liposomes wasdetermined and plotted as a function of time ... supernatant by the addi-tion of LolA, whereas essentially all Lpp moleculesremained in the spheroplasts in the absence of LolA.The addition of mLolB did not cause the release of Lpp.Taken...
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Báo cáo khoa học: "CLASSIFICATION OF MODALITY FUNCTION AND ITS APPLICATION ANALYSIS TO JAPANESE LANGUAGE" doc
... constraints. As a result, in this case, "passive" modification case frame is selected as a best matching, and case role of each case element is determined as follows : casel= Object, case2 ... representations of noun phrases or adverb phrases while the predicate has that of verb or adjective or adjective verb. A noun phrase is defined as the recursive concatenation of noun phrase or that of ... the assignment of adverbs and modal particles : (1) Among the two kinds of adverbs, namely sentence adverbs and case adverbs, we assign sentence adverbs to modality while case adverbs to case...
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Báo cáo khoa học: Impairment of mitochondrial function by minocycline pptx
... MC was addedfrom an aqueous stock solution (10 mm). Incubations wereroutinely performed at 30 °C.Measurement of swelling and cytochrome creleaseSwelling of mitochondria was measured as decrease ... Ca2+concentration was measured with CaG as Ca2+fluorochrome. Representative traces of a singlepreparation out of three preparations are shown.ABFig. 4. Effect of MC on CRC and Dwm of Ca2+-loaded ... metalloproteinases [15]. The antia-poptotic effect of MC has been attributed to upregula-tion of the antiapoptotic protein Bcl-2 [12,13], reducedexpression of caspases [8,16], and inhibition of theKeywordsmagnesium;...
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Báo cáo khoa học: Inhibition of Hsp90 function delays and impairs recovery from heat shock ppt
... and the supernatant recoveredto give a final protein concentration of 2mgÆmL)1. Pro-tein concentration was measured by Bradford Assay (Bio-Rad), and radioactivity per lg protein was measured ... absence of Hsp90 function. As a conse-quence of ansamycin pretreatment, the rate of Hspsynthesis is maintained for a longer duration and excess amounts of each of the major classes of Hspsaccumulate. ... pelletwas disrupted by pipeting. 1 ⁄ 20 volume of RNase ⁄ DNasesolution (5 mgÆmL)1DNase, 2.5 mgÆmL)1RNase A,500 mm Tris pH 7.0, 50 mm MgCl2) was added for 1 min,viscosity reduced by pipeting,...
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Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx
... GappedBLAST and PSI-BLAST: a new generation of proteindatabase search programs. Nucleic Acids Res 25,3389–3402.23 Falk A, Ek B & Rask L (1995) Characterization of anew myrosinase in Brassica ... been classified as a myrosinase-bind-ing protein-like protein. Myrosinase is a glucosinolate-degrading enzyme [4], and myrosinase-binding protein(MyroBP) has been identified as a component of high-molecular-mass ... of high-molecular-mass myrosinase complexes in extracts of Brassica napus seed [5]. The presence of threemyrosinase genes and several putative MyroBPs hasbeen reported in A. thaliana [6–8]. The myrosin-ase...
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Báo cáo khoa học: Design of expression vectors for RNA interference based on miRNAs and RNA splicing potx
... transfection and luciferase activity measured using the Dual-Luciferase Reporter Assay Systemfrom Promega (Madison, WI, USA). Luciferase activitywas defined as the ratio of firefly luciferase activity ... by polymerase II promoters arebased on endogenous small RNAs ( 22 nucleotides)known as microRNAs (miRNAs) that can also guidecleavage of RNAs and ⁄ or translational inhibition. Cul-len and ... the XhoI and EcoRIsites of the resulting plasmid. The pmiR30 used in thisstudy (containing an EGFP ORF) was generated by sub-cloning the miR30 cassette into the NheI and Acc65I sites of pEGFP-N1....
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Báo cáo khóa học: Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 ppt
... significance of the assembly of theelementary unit of fibroin in ERIt was demonstrated in this study that the elementaryunit of fibroin, having the same molar ratio of H- and L-chains, and fhx/P25 as of ... °Cfor1minandA550was measured. Fractions containing Golgi complex weremonitored by the assay for Golgi a-mannosidase II [13–15] as follows. A 15-lL portion of each fraction was mixed with135 lLof0.89mM4-metylumbelliferyl-a-D-mannopyrano-side ... fhx/P25was detected as two bands of 30 and 27 kDa (Fig. 5C, lanes3, 7 and 4, 8) like in the normal breed C108 (Fig. 5C, lanes 1 and 5). The slight decrease in electrophoretic mobility of fhx/P25 after...
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Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf
... was produced [14]. This phenom-enon was a lso d escribed for the Trametes villosa laccaseproduced in A. oryzae [6]. Glycosylation was 0.5% of the molecular mass of t he native laccase and and ... degrade the products of the cellulases [24,25].Lignin degradation occurs through the action of oxidore-ductases, such as manganese peroxidase (MnP), ligninperoxidases (LiP) and laccase. These enzymes ... with laccase signal peptide. Lessthan 1 mgÆL)1 of recombinant laccase was obtained as compared with 45 mgÆL)1 of wild-type laccase from thedikaryotic strain I-937 of P. cinnabarinus and 145...
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Báo cáo khoa học: Role of the plasma membrane leaflets in drug uptake and multidrug resistance ppt
... TMRM taken up by the cells as well as the extent of the second phase of TMA-DPH fluo-rescence quenching by TMRM (Figs 6 and 7). By con-trast to over-expression of Pgp, over-expression of MRP1 had ... addition of cells to amedium containing TMA-DPH, the fluorescence of thelatter increased by at least a factor of 100 as a result of adsorption of dye on the outer leaflet of theirplasma membrane ... accumulation of dye in thecytoplasmic leaflet of the plasma membrane, presumably as a result of flip-flop of dye across the plasma membrane and slow diffusion from the innerleaflet into the cells. Whereas...
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