Báo cáo khoa học: "Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease" pot

Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx

... of wild-type occludin (OccWT) and variant occludin in apoptosis and invasion, as determined by assay, werevealed that exon 9 played a major role in the induc-tion of mitochondria-mediated apoptosis and ... (sense) and 5Â-GAAAAAACGCGATCCTACTT-3Â (antisense). Primersfor unmethylated DNA were: 5Â-GAAGTAGGTGGAGTATTGAAT-3Â (sense) and 5Â-CAAAAAAACACAATCCTACTT-3Â (antisense).Caspase 3 activityCells subjected ... apoptosis and invasion FEBS Journal 275 (2008) 31453156 ê 2008 The Authors Journal compilation ê 2008 FEBS 3155 A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and...

Báo cáo khoa học: Roles of the SH2 and SH3 domains in the regulation of neuronal Src kinase functions pptx

... application of SH2 domain binding peptides, which results in blocking of the binding of the SH2 domain to the substrate and thereby preventing interaction of the substrate with the kinase domain. For ... between Src family kinases (SFKs), stabilized by binding of the phosphorylatedC-terminus to the SH2 domain and ⁄ or binding of the SH2 kinase linker to the SH3 domain, lock the molecules in a closed ... Y527FD1)250N-terminal, SH3, SH2, kinase domain and C-terminusDeletion of N-terminal, SH3 and SH2 domain of kinase- dead n -Src B. R. Groveman et al. A novel function of Src SH2 and SH3 domains FEBS Journal...

Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

... advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the relationship between protein stabilization by osmolytes and its con-sequent ... protein stability and activity are not largelycoupled in the presence of osmolytes. However, protein stability and activity have a linear correlation in the presence of methylamines and sugar osmolytes. ... hydration of the protein domain is the driving force of protein stabiliza-tion or folding [3,4]. Furthermore, the effect of osmolytes on the functional activity of an enzyme hasalso been investigated...

Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot

... 6E). From these results it can be concluded that tRNAswere bound in classical states with the tRNA Metfboundin the P-sites of the 30S and 50S subunits and the tRNA Phebound in the A-site of the ... Production of thisalarmone results in a down-regulation of stable RNAsynthesis (rRNA and tRNA) and up-regulation of the synthesis of mRNAs encoding enzymes involved inamino acid biosynthesis. The stringent ... [28]).Addition of a threefold excess of tRNA Pheto the tRNA Metfprogrammed ribosomes resulted in a strongFig. 5. Autoradiograms showing the template and tRNA- depend-ence of the pppGpp-synthesis...

Báo cáo khoa học: Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus inﬂuenzae pot

... Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus in uenzaeMartin Ma˚nsson1, Derek W. Hood2, E. Richard Moxon2and Elke ... Weatherall Institute of Molecular Medicine,John Radcliﬀe Hospital, Oxford, UK Structural analysis of the lipopolysaccharide (LPS) from nontypeable Haemophilus in uenzae strain 981 has beenachieved ... H-1 and H-2 (a- conﬁguration). On the basis of the chemical shift data and the large J2,3, J3,4andTable 4. Structures of the Hep4-glycoforms of nontypeable Haemophilus in uenzae (NTHi) strain...

Báo cáo khóa học: Assembly of the silk ﬁbroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1,2-mannose residues in N-linked oligosaccharide chains of ﬁbrohexamerin/P25 ppt

... Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1 ,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 Satoshi ... the assembly of the elementary unit of fibroin in ERIt was demonstrated in this study that the elementary unit of fibroin, having the same molar ratio of H- and L -chains, and fhx/P25 as of the elementary ... of L-chain in the elementary unit of fibroin (Eur. J. Biochem. 271) 363 A role of L-chain in the protection of a1 ,2-mannose residues of the N-linked oligosaccharide chains of fhx/P25 in the elementary...

Báo cáo khoa học: H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae An experimental and theoretical approach pot

... concom-itant appearance of inosine (Fig. 1A) . Incubation timeslonger than 30 min in the presence of H2O2did not greatlychange the ratio SATP + ADP + AMP/Ino. Similarchanges in ATP and inosine ... for 30 min, an important decrease in the ATP, and a less extensive decrease in the GTP, CTP, UTP and ADP-ribose levels was estimated. Concomitantly a net increase in the inosine levels was observed. ... H2O2evokes a decrease and an increase in the intracellularconcentration of ATP and inosine, respectively. Searchingfor the rationale for these phenomena, possible changes in the speciﬁc activities...

Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf

... A. niger (Eur. J. Biochem. 269) 607 Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme Eric Record1, Peter J. Punt2, ... clear band of around70 kDa was observed corresponding to the wild-type laccase from P. cinnabarinus. Immunodetection of the laccase was performed using antibodies raised against the P. cinnabarinus ... bycontinuous level of expression of the recombinant lac1transcripts during the same growth period (Fig. 4).Puriﬁcation and characterization of the recombinant laccase Puriﬁcation procedure. Recombinant...

Báo cáo khoa học: Paralog of the formylglycine-generating enzyme – retention in the endoplasmic reticulum by canonical and noncanonical signals pptx

... compilation ª 2008 FEBS Paralog of the formylglycine-generating enzyme – retention in the endoplasmic reticulum by canonical and noncanonical signals Santosh Lakshmi Gande1, Malaiyalam Mariappan1, ... pFGE by western blotting, usingan antiserum against pFGE. The total expression level and the per-centage of intracellular pFGE are given.ER retention by noncanonical retention signals S. L. Gande ... 29 5–3 01 includingPGEL are not visible in the crystal. The two calcium ions in each of the monomers are shown as gray spheres.S. L. Gande et al. ER retention by noncanonical retention signals FEBS...

Báo cáo khoa học: Role of the plasma membrane leaﬂets in drug uptake and multidrug resistance ppt

... December2009)doi:10.1111/j.1742-4658.2009.07555.x The present study aimed to investigate the role played by the leaflets of the plasma membrane in the uptake of drugs into cells and in their extrusionby P-glycoprotein and multidrug resistance- associated ... surface of the cells, flip-flop across the plasma membrane, accu-mulation of dye in the cytoplasmic leaflet of the plasma membrane and release into the cell interior. The initial binding of dye to the ... is inconsistentwith the possibility that quenching occurs as a result of dye moving within the outer leaflet of the plasma membrane. The lipid core of the plasma membrane constitutes the main...

Báo cáo khoa học: "Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis" pdf

... is to examine the expression of Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoimmune EncephalomyelitisTae-kyun Shin*, Yong-duk ... in part Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis11Fig. 1.Immunohistochemical staining of ... cells and multipotent progenitor cells. In the present study, the expression patterns of nestin and vimentin in the spinal cords of rats w ith experimental autoimmune encephalomyelitis (EAE) and the...

Báo cáo khoa học: "Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease" pot

... Agurel@istanbul.edu.trLesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia diseaseBurak Kuscu, Aydin Gürel*Department of Pathology, Faculty of Veterinary Medicine, ... Lesions in the thymus and bone marrow in chicks with CIA 21Fig. 2. (A) Abundant antigen-specific staining in the cortex and, to a lesser extent, in the medulla of the thymus section of ... SPF chicks were inoculated with the Cux-l strain of chicken infectious anemia virus (CIAV), and the clinical development of disease and its macroscopic and microscopic alterations in the thymus...

Báo cáo khoa học: "Application of light microscopical and ultrastructural immunohistochemistry in the study of goblet cell carcinoid in the appendix" pdf

... with the hypothesis of the dual ento-dermal and neuroendocrine origin of goblet cell carcinoid [1]. The ultrastructural investigation showed that in mor-phology the endocrine cells were from the ... among the groups of goblet cells infiltrating the submucosa and muscle layer of appendix. Similar clusters of goblet cells and immunorea-tive endocrine cells were also found in the wall of the ... goblet cell carcinoid [7,8]. In the last years an adjuvant chemotherapy was applied in the treatment of this type of carcinoid [9].Almost all of the studies concerning precise diagnosis of goblet...

báo cáo khoa học: " Position of the Phi and Po2 loci in the Hal linkage group in pigs" ppsx

... halothane. Two recombinants (Table 1, Original article Position of the Phi and Po2 loci in the Hal linkage group in pigsP. VögeliInsfifute of Animal Sciences, Breeding ... informative recombinants between S, Hal, Phi and H on one side and Po2 and Pgd on the other. All five recombinants are informative in determining the position of the Po2 ... 1986). The aim of this paper is to reconsider the gene order in the linkage group, especially of the Phi and Po2 loci and to establish the haplotypes (including Hal genotypes)...

Báo cáo khoa học: "Luminal concentrations of L- and D-lactate in the rectum may relate to severity of disease and outcome in septic patients" ppt

... assessment during treatment may be possible [29].ConclusionLuminal concentrations of L- and D-lactate in the rectum areincreased in septic patients and may relate to severity of dis-ease and outcome. ... concentrations of L- and D-lactate in the rectum may relate to severity of disease and outcome in septic patientsVibeke L Jørgensen1, Nanna Reiter2 and Anders Perner21Department of Anaesthesia and ... were independent of the site of infection. More importantly, these changes corre-lated to severity of disease and outcome. This indicates thatelevated luminal concentrations of L- and D-lactate...

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