... use of leucine,isoleucine, valine, methionine, tyrosine, tryptophan or phenylalanine as a sole nitrogen source, leads to theformation of the fusel alcohols and acids (Fig. 1) [1].Indeed, in Saccharomyces ... of N-terminal methionine from recom-binant proteins by engineered E. coli methionine amino-peptidase. Protein Sci13, 1802–1810.29 Duggleby RG (2006) Domain relationships in thiaminediphosphate-dependent ... was obtained by superimposingthe cofactors of several ‘HH’ motif decarboxylases,that the relative size of the substrates is inversely pro-portional to the size of the residues. Accordingly,...