Báo cáo khoa học: "Changes of biomarkers with oral exposure to benzo(a)pyrene, phenanthrene and pyrene in rats" ppt

... Changes of biomarkers with oral exposure to benzo(a )pyrene, phenanthrene and pyrene in rats 365Fig. 5. Changes of PAHs and their metabolites in urine at different time points by treatment with ... sul-Changes of biomarkers with oral exposure to benzo(a )pyrene, phenanthrene and pyrene in rats 367present study, BaP and its metabolite, 3-OH-BaP, were not detected and the concentration of PH was ... analyzed with liquid chromatography according to the method of Islam et al. [19].Determination of parent compounds in muscle and fat, and their metabolites in urine For the determination of BaP,...

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... plasmidcombinations were spotted either onto medium lacking histidine and leucine to select for the plasmids, or onto medium lacking histi-dine, leucine and uracil to monitor protein interactions. ... Thesplit-ubiquitin system. The N-terminal and C-terminal halves of ubiquitin (N-Ubi and C-Ubi) alone do not assemble. If a protein ‘X’,fused to N-Ubi, binds to the PAKs Ste20p or Cla4p, linked to C-Ubi,both ... described above) and visualized on TLC plates by staining with iodine vapor.Bands of steryl esters were scraped off, and radioactivitywas measured by liquid scintillation counting using an LSCSafety...

Báo cáo khoa học: " Validation of an elastic registration technique to estimate anatomical lung modification in NonSmall-Cell Lung Cancer Tomotherapy" docx

... correct value to be used in rela ting dosi-metric indices with treatment outcome [5]. To analyze and study the anatomical changes of lungparenchyma due to radiation therapy, and to calculate ... loss and in patient 1 because of mediastinum shift(Table 2). Furthermore patient 1 presented a mediastinumshift during the course of therapy and an increasing atelec-tasis of the left lung with ... xiis the intensity of the i - th voxel in thefixed image and yiis the intensity of the correspondingvoxel in the registered image;¯x and ¯yare the meanintensity of the fixed and the registered...

Báo cáo khoa học: Degradation of chitosans with chitinase B from Serratia marcescens Production of chito-oligosaccharides and insight into enzyme processivity docx

... of 3.04 and a FA of 0.44, and was concluded to consistmainly of DDA and DAA, in addition to minoramounts of ADA. The tetramer fraction showed a DP of 4.1 and a FA of 0.33 and the dominant tetramerswere ... by adding 5 lgofChiB and was stopped after between 15 and 10 080 min byadjusting the pH to 2.5 with 1.0 m HCl, and immersing thesamples in boiling water for 2 min. The samples werestored ... in a nonprocessive mode of action.Taking together all information and observations,ChiB is likely to initially bind the chitosan chain with similar chances of having an odd or even number of sugar...

Báo cáo khoa học: Complexes of Thermoactinomyces vulgaris R-47 a-amylase 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with a-(1,6) glycosidic linkages docx

... formfavorable interactions with the substrate. In the bind-ing of G6, Phe313 mainly interacts with the six mem-bered ring of Glc–3, while in the binding of P2, itmakes contact with the C6 atoms of Glc–2 ... Glc–1 of G6 to bind to subsite )1more deeply than that of P2. This may be caused byFig. 5. Stereoview of the catalytic site of D356N ⁄ P2. The binding of P2 is shown in green, and the binding of ... substrates and the enzyme.Domain N of TVAI is a starch-binding domain acting as an anchor in thecatalytic reaction of the enzyme. In this study, additional substrates werealso found to bind to domain...

Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx

... 5¢-end of theATT-template) folding into a hairpin-like structure. Thedouble helix of the hairpin stem, stabilized by the binding of DAPI into the minor-groove of AÆTandTÆTbase-pairs,appears to ... observablefluorescence band and a change in electrophoretic mobilitydue to the drug binding. This is attributable to the property of DAPI that favours folding of the random coil ATT strandinto a fast-migrating ... and minor-groove binding, indicating minor involvement of DNA bases [16]. However, the absence of a comparableinhibitory effect at GC-rich random sequences indicates asignificant direct or indirect involvement...

Báo cáo khoa học: Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding pot

... impact of RNA binding on pro-tein structure and substrate-binding ability.Proteins that bind and process nucleic acids areoften capable of binding to both RNA and single-stranded or double-stranded ... an intrinsic relationship between the RNAbinding and protein folding in RNA–UDE.Interestingly, treatment of RNA–UDE with RNa-se A did not result in signiﬁcant changes of the meltingcurve, indicating ... do notoffer independent support in this respect.Characterization of the nucleic acid-bindingability of UDE In order to provide a quantitative description of UDEDNA-binding and RNA-binding properties,...

Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt

... immunopre-cipitate, indicating that the interactions of these differ-ent proteins are likely to be indirect, depending on thepresence of intact RNA as integral component of theRNP.Association of 42Sp50 with ... shuttling protein with afunction in the nuclear export of localizing mRNAs. To determine whether 42Sp50 is capable of shuttling,we injected the radioactively labelled in vitro-translatedprotein ... proteins,such as the embryonic poly(A) binding protein, the Y-box RNA-packagingprotein 2B and the oocyte-speciﬁc version of the elongation factor 1a(42Sp50). Incorporation of 42Sp50 into localization...

Báo cáo khoa học: Biosynthesis of platelet glycoprotein V expressed as a single subunit or in association with GPIb-IX doc

... cells foundinefﬁcient membrane insertion of GPIba transfected as asingle-chain and secretion of a glycocalicin-like s olubleform.The exact m echanism l eading to release of GPV into t heculture ... will be important in the perspec-tive of determination of its 3-D s tructure, the mapping of ligand domains such as the sites interacting with collagen and the mechanisms of cleavage by proteases.AcknowledgementsWe ... (1999) Glycoprotein (GP)Ib-IX-transfected cells roll on a v on Willebrand factor matrixunder ﬂow. Importance of the GPIb/actin-binding protein (AB P-280) interaction in maintaining adhesion under...

Báo cáo khoa học: Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling pdf

... to treatment with JH alone.Subordination of JH III signaling through USP to status of EcR activationThere remains the question of why overexpression of EcR, leading to increased formation of ... been presented showing the ‘sub-ordination’ in vivo of RXR to the liganded state of RAR arises from the inability of RXR ligand to effectdislodging of corepressor [10]. In the RXR ⁄ VDR3 sys-tem, ... signaling is integrated through invertebratenuclear hormone-binding receptors has not been heretofore experimentallyelucidated. This report investigates the integration of signaling of inverteb-rate...

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