Báo cáo khoa học: "Primary structure of mature SAG1 gene of an Indonesian Toxoplasma gondii and comparison with other strains" pps
Báo cáo khoa học: Protein expressed by the ho2 gene of the cyanobacterium Synechocystis sp. PCC 6803 is a true heme oxygenase pot
... nitrogen nuclei both of 15NO and of 14N-proximal ligand cause the splitting. Theheme proximal ligand is considered to be His16, whichcorresponds to His17 of the proximal ligand of heme-Syn HO-1. ... characterization of the cyanobacterialHO-2 protein and its heme complex.Results and DiscussionExpression and purification of Syn HO-2Culturing the cells at two temperatures, first at 37 °C and then ... shifted from 393 to 425 nm with theappearance of a ⁄ b bands at 568 and 538 nm and absorbance at 425 nm increased. These results indicatethat the iron(III) form of myoglobin was reduced tothe...
- 11
- 347
- 0
Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt
... Kruus2,Juha Rouvinen1 and Nina Hakulinen11 Department of Chemistry, University of Eastern Finland, Joensuu, Finland2 VTT Technical Research Centre of Finland, Espoo, FinlandKeywordsascomycete; ... b-bar-rels of domains A and B (Fig. 2B), and had six hydro-gen bonds, three to the main chain carbonyls at Asn6,Leu168, and Val170, and three to the side chains of Arg11, Arg71, and Tyr216.The ... effect of hydroxide inhibition. Both TaLcc1 and MaL are alsorather thermostable as compared with many other laccases. The stabilization of both the N-termini and C-termini of TaLcc1 and MaL...
- 13
- 888
- 0
Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt
... Hospital and University of New South Wales, Sydney, NSW, Australia4 School of Chemistry and Molecular Biosciences and Centre for Infectious Disease Research, University of Queensland, Brisbane, ... importin-a recognition of theNLS and is conserved in all human CLICs, exceptCLIC3. The significance of the motif is clear, with 92% of the hydrogen bonds formed between importin-a and the CLIC4 NLS ... associates with nuclear transport fac-tor-2 and Ran [2]. The nuclear transport factor-2 path-way is normally used for the nuclear import of RanGDP [28], but nuclear transport factor-2 can alsointeract...
- 14
- 741
- 0
Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... (Fig. 3A). Three of the ligands,OD2 of Asp165, NE2 of His79 and NE2 of His169,formed an equatorial plane. The other protein ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, ... coordi-nated with six ligands: the five same ligands in theMn-reconstituted enzyme and an additional water oxy-gen, which, together with the OD2 of Asp165, theNE2 of His79 and the NE2 of His169, ... polypeptides consisted of sevena-helices, a three-stranded antiparallel b-sheet and loopsconnecting these secondary structure elements. The con-tents of the a-helix and b-strand were 50% and 11%,respectively....
- 12
- 762
- 0
Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
... crystal structures of the ligand-free PhyK at 1.7 A˚resolution and of a cata-lytically incompetent PhyK mutant that suggest amodel for substrate binding. Comparison with thestructures of the ... compilation ª 2010 FEBSmutagenesis of phyK. The H25A mutation was confirmedby sequencing analysis. Gene expression and purification of PhyKThe genes encoding PhyK and the mutant PhyK H25Awere expressed ... some characteristics with one group and other features with another.Materials and methodsCloning of the Klebsiella phytase gene (phyK)The Klebsiella phytase gene phyK was amplified using prim-ers...
- 13
- 766
- 0
Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx
... determined the structure of HtA by NMR methods. The structure consists of one a-helix, a helical turn and seven b-strands thatform an N-terminal hairpin and an anti-parallel b-sheet, with a characteris-tic ... FEBSinvolvement of a catalytic pair constituted by an acid and a base on each side of the hydrolyzed bond [16].E96 and H137 in a-sarcin and E58 and H92 in RNaseT1 act as the base and acid groups, ... 4. Comparison of the spatial orientation of the N-terminal b-hairpin and loops 2 and 5 in HtA and a-sarcin. The backbone trace isrepresented in blue for the b-hairpin, orangefor loop 5, and...
- 10
- 607
- 0
Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... showsexcellent stereochemistry, with 98.5% of all residues in themost favored and favored regions of the Ramachandranplot and r.m.s.d. values for bond and angle of 0.005 A˚ and 1.37 ° as shown in Table ... crystal structure of the complex of this protease with benzamidine.Results and DiscussionOverall structure The catalytic domain of DESC1 resembles an oblateellipsoid with diameters of 38 and 48 ... proteinase(TTSP), an expanding protein family with membersdifferentially expressed in several organs and certaintumors. To date, more than 30 mammalian members of this group have been identified and have,...
- 13
- 588
- 0
Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc
... hydrogen-bon-ded antiparallel b-strands (b6–b7), an a-helical hairpin(helices a5, a6) and a C-terminal strand (b8). Helix a5 and strand b7 partially cover one side of the Ross-mann fold structure, ... three-stranded b-sheets, each one consisting of the antiparallel b-strands b6, b7 of one monomer and strand-b8 of the other. Solvent-accessible surface [15]calculations show that a substantial ... Asp14 and Arg140) and on strand b8 which plays a role onoligomerization and thus probably affects enzymeactivity and substrate specificity.Experimental procedures Structure determination and...
- 11
- 710
- 0
Tài liệu Báo cáo khóa học: Trichostatin A reduces hormone-induced transcription of the MMTV promoter and has pleiotropic effects on its chromatin structure pptx
... B area,protection of the nucleosome C area and repositioning of initially randomly positioned nucleosomes (Fig. 5A and B,compare lanes 1, 2, and 3, 4 and corresponding scans). Quiteunexpectedly, ... TSAadministration has on chromatin structure and on gene expression.Materials and methodsDNA and plasmidsConstruction of the MMTV reporter and the plasmid forin vitro transcription of rat GR mRNA has been ... low level of constitutive transcription. This correlates with a change of acetylation pattern and a more open chromatin structure over the MMTV chromatin, and with specific acetylation and remodeling...
- 10
- 500
- 0
Từ khóa: Nghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015HIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP