Báo cáo khoa học: "Experimental reproduction of proliferative enteropathy and the role of IFN-gamma in protective immunity against Lawsonia intracellularis in mice" pdf

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... GGGTTCT AACAAGGGTGCT-3Â;Abc, 5Â-CACAACGCCACCAACCATCAGACCGATGATAGCACCCTTGTTAGAACCCAC-3Â;Ab-start, 5Â-GCGTAGGGTCGACATATGGACGCTGAATTCCGTCACG-3Â;Abstop, 5Â-CCTGCCGAGCTCCTATTACACAACGCCACCAACCATCAG-3Â. The ... according to the manufacturer’s guidelines and using the following primers: Aba, 5Â-ATGGACGCTGAATTCCGTCACGACTCTGGTTACGAAGTTCACCACCAGAAGCTGGTG-3Â;Abb, 5Â-GTTCACCACCAGAAGCTGGTGTTCTTC GCTGAA GACGT GGGTTCT ... SEC-isolated Ab(1–40) and Ab(M1–40) are at least 97%pure. In the lanes containing Ab(1–42) and Ab(M1–42), there were prominent bands at approximately4 kDa and faint bands at approximately 14 kDa....

Tài liệu Báo cáo khoa học: An engineered disulﬁde bridge mimics the effect of calcium to protect neutral protease against local unfolding docx

... FEBS An engineered disulﬁde bridge mimics the effect of calcium to protect neutral protease against local unfolding Peter Duărrschmidt*, Johanna Mansfeld and Renate Ulbrich-HofmannDepartment of ... Model of the 3D structure of the neutral protease from Bacillus stearothermo-philus, including the position of the disulﬁde bridge in mutant G8C ⁄ N60C. The modelwas created on the basis of the ... act as an indicator for the occurrence of the intermediate in the unfolding. Calcium ions are the main contributory factor to enzyme stability The binding of calcium ions seems to be the mainsource...

Tài liệu Báo cáo khoa học: "From HOPE en I''''ESPERANCE On the Role of Computational Neurolinguistics in Cross-Language Studies " pptx

... meaning. It is the in interaction of the results of these asynchronous processes that the process of comprehension is defined. The processes are independent of the know- ledge representations ... activation of all meanings of each recognized word in HOPE, the determination of the phonetic representation of a recognized word determines the breadth of active competition amon 9 meanings ... time depending on the input, interconnections to other information, and an automatic activity decay scheme. In addition, the decay scheme is based on the state of the information, whether it...

Tài liệu Báo cáo khoa học: "Language Resources Factory: case study on the acquisition of Translation Memories" potx

... Association for Computational LinguisticsLanguage Resources Factory: case study on the acquisition of Translation Memories∗Marc PochUPF Barcelona, Spainmarc.pochriera@upf.eduAntonio ToralDCU ... this case only the WSP needs to have a deepknowledge of the installation and maintenance of the tool, thus allowing all the other users to beneﬁt1 6 Evaluation The evaluation of the factory ... proof of concept that thesetechnologies can grow and scale to beneﬁt manyusers.7 Case study We introduce a case study in order to demonstrate the capabilities of the platform. It regards the...

Tài liệu Báo cáo Y học: The effects of low pH on the properties of protochlorophyllide oxidoreductase and the organization of prolamellar bodies of maize (Zea mays) pot

... form of a phosphorylated (or possibly adenylated) ternary complex of POR, its substrate PChlide, and NADPH. Dephospho-rylation of the photoconverted form of this complex by anendogenous phosphatase, ... 12B. The effect of the presence of the adenylates on the ability of POR-PChlide640to undergophotoconversion was checked by comparing the efﬁciency of photoconversion of PLB samples containing ... currentsuggestions of the possible involvement of phosphorylation(or adenylation) in changes in the aggregational state of the POR complex.Keywords: protochlorophyllide oxidoreductase; prolamellar body; protochlorophyllide; ...

Báo cáo khoa học: A novel 2D-based approach to the discovery of candidate substrates for the metalloendopeptidase meprin pot

... mayspeculate that hmeprin has activity similar to BMP-1 ⁄TLD-like metalloendopeptidases in that it acts as a procollagen C protease as well as an activator of lysyloxidase. Therefore, an important ... residues, vari-able oxidation of methionine and variable deamidation of asparagine and glutamine. Parent and fragment mass toler-ances were set to 1 Da. Up to two missed cleavages andhalf tryptic ... PAGE-based image analyses of the second quadrant.Fig. S2. 2D IEF ⁄ SDS ⁄ PAGE-based image analyses of the third quadrant.Fig. S3. 2D IEF ⁄ SDS ⁄ PAGE-based image analyses of the fourth quadrant.This...

Báo cáo khoa học: Puriﬁcation, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

... primers designed from the cDNA sequenceresulted in the amplification of bands in each case. Sequencing of these bands showed that they matched the sequence of the cellulase cDNA completely; however, ... conditions[4,18,19]. The optimal pH for the purified cellulase from the larval gut of P. hilaris against CMC was also 5.5. This isreasonable for the physiological function of cellulase activityin larval guts as ... PCR and sequencing A QuickPrepÒ Micro mRNA Purification Kit (AmershamBioscience) was used for isolation of mRNA from P. hilaris larval midguts. First-strand cDNA synthesis from the isolated mRNA...

Báo cáo khoa học: "Last but Definitely not Least: On the Role of the Last Sentence in Automatic Polarity-Classification" pdf

... 2010.c2010 Association for Computational Linguistics Last but Definitely not Least: On the Role of the Last Sentence in Automatic Polarity-Classification Israela Becker and Vered Aharonson AFEKA – ... experiment we examined the readers’ rating of the same re-views based on reading single sentences ex-tracted from these reviews: the last sentence or the second one. The second sentence could ... for last sentence only (b), and for second sentence only (c). 334 of paragraphs they were asked to read by rely-ing on sentences in initial positions. These find-ing subsequently gained...

Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx

... role of Itk in T- cell development. In the absence of Itk, there is a partial block in the development of ab T cells and a reduced ratio of CD4 to CD8 single positive thy-mocytes in both the thymus ... from conventional ab T cells. This minireview focuses on this aspect of Itk, its role in the development and function of iNKT and NKT- like cd T cells. Itk and i NKT cell development NKT cells are ... asalternative modes of development. The data that areaccumulating suggest that the role of Itk in the devel-opment and function of iNKT cells and cd T cells seems to be quite different from...

Báo cáo khoa học: " Risk factors for retained placenta and the effect of retained placenta on the occurrence of postpartum diseases and subsequent reproductive performance in dairy cows" potx

... (2005),/6(1), 53–59 Risk factors for retained placenta and the effect of retained placenta on the occurrence of postpartum diseases and subsequent reproductive performance in dairy cowsYeon-Kyung Han, ... several reproductive factors in individual cows, and to determine the effects of retained placenta on the occurrence of postpartum diseases and subsequent reproductive performance in dairy herds. The health ... illhwa@cbu.ac.kr Retained placenta in dairy cows: Risk factors and effects 55 placenta among individual farms were compared using the chi-square test.Evaluation of the effect of retained placenta on the occurrence...

Báo cáo khoa học: "Experimental reproduction of proliferative enteropathy and the role of IFN-gamma in protective immunity against Lawsonia intracellularis in mice" pdf

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Báo cáo khoa học: "morphological characteristics, root growth potential and flushing response of rooted cuttings compared with transplants of Sitka spruce" pps

... between 15 and 22 °C. Original articleThe morphological characteristics, root growth potential and flushing response of rooted cuttings compared with transplants of Sitka spruceConor ... in root growth potential and flushing response of rooted cuttings derived from selected material compared with unim-proved transplants of Sitka spruce grown in Ireland. ... variables, root growth poten-tial and dormancy intensity of planting stock raised from rooted cuttings were assessed and compared with those of conventional transplants grown...

Báo cáo khoa hoc:" VEGF receptors on PC12 cells mediate transient activation of ERK1/2 and Akt: comparison of nerve growth factor and vascular endothelial growth factor" doc

... expression of VEGF receptors on PC12 cells, exogenous VEGF 165 had no effect on PC12 cell pro-liferation and neurite formation. One reason might beendogenous VEGF- expression of proliferating PC12 ... 1 of 6(page number not for citation purposes)Journal of Negative Results in BioMedicineOpen AccessResearch VEGF receptors on PC12 cells mediate transient activation of ERK1/2 and Akt: comparison ... not shown). VEGF induces transient activation of ERK1/2 and Akt kinase in differentiated apoptotic PC12 cells While the addition of VEGF 165 had no effect on PC12 cellproliferation and neurite...

báo cáo khoa học: " Coerced addiction treatment: Client perspectives and the implications of their neglect" pps

... cited.Review Coerced addiction treatment: Client perspectives and the implications of their neglectKaren A UrbanoskiAbstractRecent work has criticized the evidence base for the effectiveness of addiction ... excluded, asthey are out of the scope of the present review. Thisincludes studies of the effectiveness of applying sanctions and rewards throughout treatment, and the impact of legal or other third-party ... made of publication and library catalogues of the author's home institutions (the Centre for Addic-tion and Mental Health and the University of Toronto) and other substance policy and research...

Báo cáo khoa học: "A Clinical review: Molecular mechanisms underlying the role of antithrombin in sepsis" docx

... circulatingantithrombins are glycosylated at four of their asparaginemolecules; these are of the α isoform. The remaining 5–15% of circulating antithrombins, of the β isoform, lack glycosy-lation at asparagine ... effect in a rat model by intravital videomicroscopy of damage to the small intestine in endotoxinaemia. In both of these studies, the effect of antithrombin on the leucocyte–endothelium interaction ... ST, Bjork I: The oligosaccha-ride side chain on Asn-135 of alpha -antithrombin, absent in beta -antithrombin, decreases the heparin affinity of the inhibitor by affecting the heparin-induced conformationalchange....

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