Báo cáo khoa học: "Molecular analysis of hprt mutation in B6C3F1 mice exposed to ozone alone and combined treatment of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and/or dibutyl phthalate for 32 and 52 weeks" ppsx

... suchdominant transversion may be responsible for toxicity and combined exposure to ozone, NNK, and DBP inducesadditive genotoxicities compared to ozone alone. Key words: Ozone, NNK, DBP, hprt mutation Introduction Ozone ... 1. DNA sequence analysis of hprt mutant in splenic cells of B6C3F1 male mice in 52- wk studyType of mutation Number of mutantsControl Ozone NNKDBP** Ozone+ NNK Ozone+ DBP* Ozone+ NNK+DBPBase ... of total clones.-2851$/ 2)9H W H U L Q D U \ 6FLHQFHJ. Vet. Sci. (2004),/5(4), 379–385Molecular analysis of hprt mutation in B6C3F1 mice exposed to ozone alone and combined treatment...

Báo cáo khoa học: Molecular analysis of the interaction between cardosin A and phospholipase Da Identiﬁcation of RGD/KGE sequences as binding motifs for C2 domains pdf

... RC, Davis AF & Tornehl CK(1998) Delineation of the oligomerization, AP-2 binding, and synprint binding region of the C2B domain of synaptotagmin. J Biol Chem 273, 329 66 329 72.51 Nakatani ... This amino-acid motif mimics RGD in terms of charge and is positioned at the tip of a loop and istherefore reminiscent of RGD sequences present in integrin-binding molecules because of its exposed ... a protein-binding domain in addi-tion to its role in Ca2+-dependent phospholipid bind-ing [54]. It remains to be established if this new roleas an RGD-binding domain is exclusive to the...

Tài liệu Báo cáo khoa học: Mosquito (Aedes aegypti ) aquaporin, present in tracheolar cells, transports water, not glycerol, and forms orthogonal arrays in Xenopus oocyte membranes docx

... expressed in these serotonin receptor-expressing tracheolar cells and if serotonin is in any wayinvolved in modulating water transport in tracheolar cells.Hormonal regulation of mammalian AQP1 and ... 20mininPBSTGandincubated in a 1 : 750 dilution of biotinylated anti-rabbit IgG (VectorLaboratories) for 1 h. Tissues were washed 4 · 20 min in PBSTG and incubated in a 1 : 200 dilution of Texas ... Red-Streptavidin (Vector Laboratories) for 30 min. Tissues werewashed 6 · 30 min in PBSTG. Tissues were kept in PBSTGovernight at 4 °C and mounted in Vectashield MountingMedium with DAPI for nuclear...

Báo cáo khoa học: "A Re-examination on Features in Regression Based Approach to Automatic MT Evaluation" pdf

... Albrecht and Rebecca Hwa. 2007. A Re-examination of Machine Learning Approaches for Sentence-Level MT Evaluation. In Proceedings of the 45th Annual Meeting of the Association of Com-putational Linguistics ... Simon Corston-Oliver, Michael Gamon, and Chris Brockett. 2001. A machine learning approach to the automatic evaluation of machine translation. In Pro-ceedings of the 39th Annual Meeting of the ... for evaluation of machine translation. In ACL 2005 Workshop on Intrinsic and Extrinsic Evaluation Measures for Machine Translation and/ or Summari-zation, pages 25 -32, June. Christopher B. Quirk....

Báo cáo khoa học: Molecular cloning, expression analysis and functional conﬁrmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf

... difference in receptor-bindingafﬁnity of DBHs among insect orders might ratherbe attributed to the amino acid residues which areconsidered to be involved in binding to both types of ligands. Amino ... successfully performed cDNAcloning of EcR and USP of L. decemlineata, and in vitro translation of corresponding receptor proteins.The translated proteins could bind to EcREs and ecdysone agonists ... structures of molting hormone receptorsamong insects is increasing for a better understanding of the function of molting hormone in insect growth and development.Previously, we performed structure–activity...

Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identiﬁes a calcium sensitive region required for basal activity doc

... MGP and all other members of this vitamin K-dependent proteinfamily can bind to mineral and, in particular, calcium-containing-mineral such as hydroxyapatite [2].Although the exact m ode of ... the fragments of interest (spanningthe regions )648 to +44, )464 to +44, )185 to +44, )949 to )326 , and )949 to )708, respectively) were blunt endedandinsertedattheSmaI site of pGL2LUC. The ... uggested the presence of speciﬁc binding sites for nuclear factors involved i n the regulation of MGP genetranscription in the )70/)36 region. Binding of A6 nuclearprotein(s) to this region was...

Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

... RNase-freewater. Single-stranded RNAs were allowed to anneal bymixing equal amounts of each strand, heating to 100 °C for 1 min, and cooling gradually to room temperature for 3–4 h. Single-stranded RNAs and ... including the fore gut site. Recently,CHH was reported in the endocrine cells of the foregut and hind gut of Carcinus immediately prior to and during molting, which is responsible for water uptakeat ... expression pattern to the one observed in stomach. In order to analyze the efﬁciency of gene silencingby direct injection of dsRNA into adult shrimps, wesynthesized dsRNA corresponding to CHH maturepeptide...

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... optimization for glyphosate activity is accompanied by improved bind-ing to longer ligands (up to five atoms in the mainchain) and retained binding to shorter ligands [55]. Of the 21 changes in the ... Gene shuffling improved thedesigned enzyme 200-fold to 400-fold [65], illustratingthe advantage of combining tools for enzyme optimiza-tion. With the increasing demand for food and biofuel,all ... restored by muta-tion of Ala100 to glycine, allowing glyphosate to bind in its extended, inhibitory conformation.Detoxification of glyphosateDetoxification of the glyphosate molecule is anotherstrategy...

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... overexpression, parkin and PTEN-induced putativekinase 1 loss -of- function and UCHL1 mutation, lead to an impairment of neuronal dopamine homeostasisby interfering with the vesicular storage and releasemechanisms. ... [1–6].Single point mutations in a-synuclein, as well asduplication and triplication of the gene, were reported to be linked with rare familial forms of PD [6].However, a-synuclein deposition into ... function of a-synuclein is poorly under-stood and a-synuclein expressed at low levels appears to be neuroprotective and anti-apoptotic, indicating adual role for this protein [7–9]. Several lines of...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... blot analysis was performed to investigatethe expression profile of the BmIVD gene. For bothwild-type and sku strain, a single band of 1.35 kb, cor-responding to the predicted molecular size of ... cycling consisted of 40 cycles of denaturing at 94 °C for 30 s, annealing at 54 °C for 30 s and extension at 72 °C for 90 s.5¢- and 3¢-rapid amplification of cDNA ends(RACE) In order to examine ... Theconstruction and propagation of recombinant AcMNPVswere performed according to the manufacturer’s instruc-tions (Invitrogen).Expression and purification of recombinantBmIVD proteinMonolayers of Sf9...

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