Báo cáo khoa học: " Effect of fixation time and freeze-thaw cycles on the molecular analysis of viral DNA" ppt

Báo cáo khoa học: "Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis" pdf

... EAE lesions [3]. The latter focused on the remyelination capacity of the CNS after an EAE attack.Consequently, we postulate that glial elements along the central canal and subpial regions are ... paralysis, and finally, spontaneous recovery. These steps are matchedby an inflammatory response in the CNS, which is cha-racterized by the infiltration of T cells and macrophages, and the activation ... Shin, Yong-duk Lee and Ki-bum Simnestin, vimentin and GFAP in EAE lesions in the spinalcords of Lewis rats to evaluate the activation capacity of brain cells during autoimmune inflammation.Materials...

Báo cáo khoa học: "Improved survival following surgery and radiation therapy for olfactory neuroblastoma: analysis of the SEER database" pdf

... Though the etiology is unknown[2], ONBappears to arise from the olfactory membrane of the sinonasal tract and prefere ntially involves the anatomicdistribution of the epithelium overlying the ... analysis. MEP, TLM, MM, and AKS wrote the manuscript. All authorsreviewed the statistical analysis results, contributed to the interpretation of the results and read and approved the final manuscript.Competing ... available).Platek et al . Radiation Oncology 2011, 6:41http://www.ro-journal.com/content/6/1/41Page 5 of 7ConclusionsThis analysis of the largest series of ONB cases from the SEER database suggests,...

Báo cáo khoa học: " Effect of fixation time and freeze-thaw cycles on the molecular analysis of viral DNA" ppt

... ...

Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

... affect the association of the substrate with the enzyme in anyone of several ways, e.g. through solvation effects on substrates or enzyme active sites and through theireffects on the thermodynamic ... understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the relationshipbetween protein stabilization by osmolytes and its con-sequent effects ... have little or no effect on the solvation properties of the substrate and the enzyme active sites or on theirthermodynamic activities. Another explanation forthese observations comes from our...

Báo cáo khoa học: Endogenous tetrahydroisoquinolines associated with Parkinson’s disease mimic the feedback inhibition of tyrosine hydroxylase by catecholamines doc

... formation of hydrogen bonds between the nitrogen atoms of the ligands and the backboneoxygen of Pro325. Although we did not directly com-pare the molecular interaction energies of NMTIQ and MPTP ... center of the catalytic site, the latter prompting oxidation of the iron and consequenthTH inactivation. TH inhibition by endogenous TIQsdepends critically on 6,7-dihydroxylation of the ben-zene ... electron paramagnetic resonance(EPR) spectroscopy, we examined the oxidation status and spin of the iron in hTH in the presence of DA and four structurally distinct TIQs. The divalent iron of the...

Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35ﬁTyr mutant pdf

... any hydrogen bond with the proteinatoms. The former propionate displays the conservedconformation in the structures of the Phe35fiTyr mutant and of the wild-type protein as well as other mutants.However, ... chainconformation of this residue. The shift of the Ca atom of Tyr35 of the Phe35fiTyr mutant from that of Phe35 of the wild-type cyt b5is 0.21 A˚, within the error limit. The sidechain of Tyr35 ... resultsdemonstrate that the Phe35fiTyr mutant increases not only the affinity of the heme to the polypeptide chain but also the stability of the secondary structure. The kinetics of the heme dissociation...

Báo cáo khoa học: A new rice zinc-ﬁnger protein binds to the O2S box of the a-amylase gene promoter pptx

... of three copies of the O2S domain at the 5¢ end of the CYC1 mini-promoter region. Following transfor-mation of the URA-marked plasmid pLGD-265 UP1containing the O2S domain and the TRP-marked ... transcription [4,5]. Rogers and Rogers[4] found that both GARE and O2S functioned only whenpositioned in one orientation with respect to each other and with respect to the TATA box, and when the distancebetween ... speciﬁcally to the O2S elemen t. Wehave also determined the importance of the amino acidswithin the binding domain of RAMY protein and analyzed the time course for the induction of RAMY a nd...

Báo cáo khoa học: Cytosolic phospholipase A2-a and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus pdf

... phospholipase A2 is in the nucleus of sub-confluent endothelial cells but confined to the cytoplasm of confluent endothelial cells and redistributes to the nuclear envelope and cell junctions upon histamine ... cell. The subcellular locations of these proteins also vary, withsome being present in the ER, others in the nuclearmembrane and some present at both these locations.Thus the relocation of cPLA2-a ... areas of colocalization.Secondary-only controls and controls in which goatpolyclonal antibody was followed by anti-rabbit and vice versa were blank.Relocation of cPLA2-a occurs to both the...

Báo cáo khóa học: CD38 is expressed as noncovalently associated homodimers on the surface of murine B lymphocytes doc

... suggest that the stabilization of CD38 homodimers by crosslinkers depends strongly on the conformation and orientation between the CD38monomers.It has been reported that NP-40 and Triton X-100stabilize ... values of each of the clones). The relative enzyme activity of each of the mutants is given in parentheses. It was determined by setting the Vmaxadjustedactivity of CD38-WT to 100% and then ... expression of CD38 on the plasma membrane and the half-lives of these CD38 mutant proteins on the plasma membrane were signiﬁcantly reduced. Together,these results show that CD38 is expressed as noncova-lently...

Báo cáo hóa học: " Research Article Real-Time Cardiac Arrhythmia Detection Using WOLA Filterbank Analysis of EGM Signals" pot

... representing the beat locations. By further analysis of the synchrony of the subband beats and the periodicity and regularity of the optimal beat, various possible cardiac events (in-cluding ﬁbrillation, ... signal, the narrowband (com-plex) subbands are merged to obtain wideband-subband sig-nals to be used for wideband event detection. The results of the wideband and narrowband detections are then ... Subband peaks are combined in parallel by exploiting the synchrony of the subband signals at the beat time. Fur-thermore, to cope with the wide range of possible beat rates and morphologies of the...

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