Báo cáo khoa học: "Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System" ppsx
Báo cáo khoa học: "Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System" ppsx
... Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System 75 a two-step culture system. Development to blastocyst in medium containing ... alwayscomprised of a single formulation for all stages [20]. Manyhave investigated the in vitro culture of bovine embryos, but the relation between metabolic changes and developmentalstages remain unclear. ... 33342 stain prepared withsodium citrate (2.3%) and ethyl alcohol was added. The slidewas then incubated on a warming plate for 5 min, the extrastain was discarded, and Permount was added along...
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Báo cáo khoa học: MAP2 prevents protein aggregation and facilitates reactivation of unfolded enzymes ppt
... MAPs are again a mixture of proteins containing predominantly MAP2 and tau withtraces of MAP1, MAP4, etc. [33]. We checked the Fig. 1. Insulin and ADH aggregation in the presence of MTP. (A) Aggregation ... patches in MAP2 and tau, it is noted that they contain 18.6 and 12.7% acidic amino acids, respectively, indicating both of them are predominantly basic in nature. In tau, the acidicamino acids ... the chaperone machinery of MAP2. Again MAP2c, despitelacking a significant portion of the N-terminal domain of MAP2, was also capable of exhibiting chaperone-likeactivity. However, MAP2c was less...
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Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx
... Mizoguchi A, Ishizaki H, Nagasawa H, Kataoka H,Isogai A, Tamura S, Suzuki A, Fujino M & Kitada C(1987) A monoclonal antibody against a synthetic frag-ment of bombyxin (4K-prothoracicotropic ... mori, as in many other holo-metabolous insects, the basal external structure of adult appendages such as wings, legs and antennaehave already been built at the time of pupation,although they are ... immature. The imaginaldisks or rudiments of the adult appendages graduallygrow and develop beneath the epidermis throughout the larval stages, and rapidly grow and evaginate at the time of...
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Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant pdf
... can also maintain the edge-to-face interaction, provi-ding the stabilization effect of the heme binding. In the case of the Phe35fiLeu mutant, however, substitution of leucinefor phenylalanine ... concentration of protein could cause denaturation of the apo -protein during the long assay time, all of the heme transfer reactions weremonitored only by the UV–visible spectra. The rates of heme transfer ... refinement at the CNSrefinementstage. A random sample of 10% of the X-ray data wasexcluded from the refinement and was taken as the test dataset, and the agreement between the calculated and observedstructure...
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Báo cáo khóa học: CD38 is expressed as noncovalently associated homodimers on the surface of murine B lymphocytes doc
... using a FACSCalibur (Becton-Dickinson, San Jose, CA, USA).Surface biotinylation of proteinsTo analyze the stability of CD38 on the plasma membrane of the different Ba/F3 mutants, labeling of ... USA). Protein concentration was determinedwith the BCA protein assay (Pierce). The catalytic activity of CD38 in Ba/F3 cell homogenates was determinedby HPLC using the radiolabeled substrates ... Panel on Euthanasia of the AVMA and in compliance with the CINVESTAV and Trudeau InstituteIACUC guidelines. The IL-3 dependent murine pro-B cellline, Ba/F3 (a generous gift from D. Campana,...
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Báo cáo khoa học: Plasmodium falciparum hypoxanthine guanine phosphoribosyltransferase Stability studies on the product-activated enzyme potx
... some preparations of the enzyme after activationcould be traced to the presence of a contaminatingphosphatase activity in these preparations. The addition of fresh IMP to these preparations restored ... rates of the unactivated enzyme. Surprisingly, acti-vation was observed only upon incubation with PRPP and hypoxanthine, and not with guanine and xanthine, the other purine substrates of the enzyme. ... investi-gation. It is also possible that PfHGPRT may be asso-ciated with other cellular proteins in vivo, allowingsubstrate channeling with the association maintaining the protein in the active conformation....
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Báo cáo khoa học: Effects on protease inhibition by modifying of helicase residues in hepatitis C virus nonstructural protein 3 pot
... zincatom in the protease domain and a phos-phate group in the helicase domain are alsovisible. The interface region between the helicase and the protease domains isframed and shown in detail in ... [8–13].Nevertheless, we are interested in determining the functional importance of the helicase domain for the activity and inhibition of the protease, and havefocused on the identification of specific helicase resi-dues ... clearwhy the two enzymes are fused into a single protein. The active sites of each of the enzymes are located in the respective domains, and truncated variants of NS3, containing either the...
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Báo cáo khóa học: Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis doc
... reading frame.Amino acid numbering starts from the N-terminal amino acid.Resistance level determined after a 24 h incubation on LB agar.StrainPosition of mutation in rpsLAmino acidreplacementResistance ... MBq, Amer-sham), and was incubated at 37 °C for 15 min. The background value was obtained from the reaction in the absence of poly(U). After the incubation, a 15 lL aliquot of the reaction mixture ... The streptomycin-resistance mutations in the ribosomal proteinsS4 and S5 confer ribosomal ambiguity (ram) phenotypes, and cause a decrease in the translational accuracy [7,8]. In the 1980s, mutations conferring streptomycin...
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Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf
... that induce apoptosis, includingUV- and c-irradiation, heat shock, protein synthesisinhibitors, DNA-damaging agents and the proinflam-matory cytokines, are potent activators of JNK.Several anti-neoplastic ... 91–118.19 Hibi M, Lin A, Smeal T, Minden A & Karin M (1993)Identification of an oncoprotein- and UV-responsive protein kinase that binds and potentiates the c-Junactivation domain. Genes Dev ... prepared at the indicated time points after the AG1478 addition and analyzed forcaspase 3 activity by using a fluorometric substrate-based assay.Each point is the mean of triplicate samples, and...
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Tài liệu Báo cáo khoa học: Effects of sequestration on signal transduction cascades docx
... types and organ-isms (Table 1). Each of these kinases modifies itstarget protein and is itself a target for the upstreamkinase. Because the concentration of kinases and theirtarget proteins are ... reduced and the upstream kinases of MAPK and MAPKK are only slightly activated and can sequester only limited fractions of MAPK and MAPKK.Discussion The function of the signal transduction network ... the enzymes are product sensitive. Data about protein abundance in signal transduction cascades are now in hand, showing that members of the cascades are pre-sent in concentrations of the same...
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