... to
investigate the expression and antioxidant effects of
PHB in pancreatic b -cells exposed to ethanol.
Results
Effects of ethanol and recombinant PHB on b-cell
mitochondrial function and apoptosis
We first ... glucose
intolerance and it may be a mechanism of pancreatic
b-cell dysfunction in type 2 diabetes, as b -cells are very
sensitive to oxidative stress due to their insuffici...
... targeted to the degradation pathway and the
130 kDa form can be recycled back to the surface.
Therefore in this study, we have described a novel means
of regulation of a member of the guanylyl cyclase ... its ligands, when present on the plasma membrane of
intestinal cells. This may partly account for the differential
responsiveness of various regions of the intest...
... values of fresh leaves, dried leaves and whole plants were 2428, 1851, and
1992 Kcal/kg DM, respectively; that of PDI values 152.3, 126.4, and 127.1 g/kg DM, respectively. Results of the
study ... leaves. In vivo digestibility of OM, CP, and NDF of fresh leaves was
78.5, 80.9 and 82.6%, respectively; those of dried leaves and whole plants were 63.1, 60.6, 76.1% and 66.1, 75....
... lgÆmL
–1
ampicillin and 50 lgÆmL
–1
chloramphenicol. A
single transformant colony (10
8
cells) was diluted with
Luria–Bertani media to 10
7
cells in 1 mL of Luria–
Bertani and 100 lL batches of the diluted cells ... 3 binds to the substrate-binding site of the
protease.
In the first experiment, we compared the
15
N-HSQC
spectra of WNV NS2B–NS3pro(K96A) in the presenc...
... consists of all 74,024 sentences
in the Wall Street Journal, Brown and ATIS Cor-
pus subparts of the Penn Treebank III. We ran-
domized the order of the sentences in this dataset,
and then split ... charged .”
stances and , ,
where is the number of features, is a weight for
feature , and estimates the difference between the
two instances’ values at the th feature. The...
... as an esti-
mation of the bandwidth and the intensity of the bands
making up the amide I or I¢ region. Thereafter, curve fitting
was performed and the heights, widths and positions of
each band ... this study we used IR spectroscopy to study the
secondary structure of the catalytic domain of PKCf
and the effect of its substrate, MgATP, and also to
study the...
... USA) and quantified using
image quant 4.1 software.
Analysis of kinetic data and determination
of apparent K
m
and k
cat
values (K
m
¢ and k
cat
¢)
For 3¢-processing kinetic study, the amount of ... product. The stability of
IN during the time-course of the reaction and the
influence of protein aggregation are discussed.
Results
General features of the 3¢-end processi...
... what Streat and coworkers [15] termed, the large
risk of unacceptable badness’, rather than a vanishingly small
potential for benefit.
There are far worse things than death, and many of them occur
in ... family members of the dying experience of older and seri-
ously ill patients. SUPPORT Investigators. Study to Under-
stand Prognoses and Preferences for Outcomes and Risks of
T...
... of Sry is translated into cellular structure
and organ morphology. Interestingly, Dmrt1, the
ancestor of Dmrt1bY, is one of the downstream effec-
tors of Sry in the male pathway.
In medaka, the ... well
studied in the context of gonadal induction and main-
tenance, illuminating its important function. But what
about the other dmrt genes? Table 2 summarizes the
expressi...
... fragment accompanied by the appear-
ance and an increase in the 80 kDa fragment (Fig. 2).
Although both the untransfected cells and the cells
transfected with the vector alone started to show the
80 ... Apoptotic cells were
identified by the condensation and fragmentation of their
nuclei. The percentage of apoptotic cells was calculated as
the number of apopto...