... enrichment in iNOS activity
within the particulate fraction of epithelial cells.
Apical distribution of iNOS in intestinal epithelial
cells
To get more insight into the localization of iNOS in
intestinal ... [18].
Results
Subcellular distribution of iNOS protein and
activity in vitro
The distribution of iNOS protein in the cytosol and
particulate fraction was exa...
... tropomyosin
and actin, the IANBD probe retained its typical
responses to changes in Ca
2+
and S1 binding (Fig. 6).
Fitting binding curves to obtain binding parameters
is difficult in the case of Ca
2+
because ... the measured
effects of Ca
2+
on binding in the presence of ATP
[35,36], equilibrium binding in the presence of ADP
[16], binding kinetics [15,37] and the genera...
... (pseudo-coloured green). C, G
andKaremergedimagesfromAandB,EandFandIandJ,respectively.AnenlargementoftheboxedareasinC,GandKareshowninD,Hand
L, respectively. Images shown in this figure are single xy confocal ... microdomains (GEM) of the plasma membrane
and to be involved in the regulation of epidermal growth
factor receptor (EGFr or ErbB1) activity. To gain further
insight into the...
... by a machine and they are of limited
variety, so that their listing in a dictionary is
practicable.
From the practical point of view, the distri-
bution is useful in planning input and storage ... not
shared by the others, of being concentrated at
relatively low values of length, and of having
no elements exceeding a certain length (Fig. 1).
Words, defined in this...
... 1).
Results
Binding of AMA and odorants in solution
The dissociation c onstants of the AMA–OBP c omplexes fo r
the bovine and porcine OBP isoforms were, respectively, 1 .0
and 1.5 l
M
and the corresponding ... onformational changes
involving protein loops or backbone. T hus, the role of OBP
in signal triggering remains unresolved.
3
Keywords: crystal str ucture; domain swa...
... strains of Japanese
encephalitis virus, 28 strains of St Louis encephalitis virus,
172 strains of West Nile virus and 24 strains of yellow fever
virus using BLASTn [22]. The genome sequences of the
flaviviruses ... to
dengue virus when computationally tested against 291
strains of other nondengue flaviviruses, including strains
of Japanese encephalitis virus, St. Louis...
... counterstaining.
Haematoxylin–eosin staining
Haematoxylin–eosin staining was carried out following
the routine histological method described by Kayser and
Bubenzer [38]. The haematoxylin–eosin staining technique
employs ... KH
2
PO
4
and 8 mm Na
2
HPO
4
, and kept at 37 °C for 15 min in a
beaker containing NaCl ⁄ P
i
. The intestine was then filled
with a solution containing 1.5 mm EDT...
... determine the calcium-binding domain of
the protein. (A) Schematic drawing of the recombinant proteins. Six
GST-fused recombinant proteins containing the three distinctive
domains of tandem repeat 1 ... Of these recombinant
proteins, rOMM-64-I, III, IV and V, which include the
Glu-rich domain, were found to have calcium-binding
activity. rOMM-64-II and -C and GST were stained
re...
... DNA and AdoHcy(AdoMet) [44], M.HhaI
consists of two domains, the large domain containing
the S-adenosyl-l-methionine (AdoMet) binding site
and the catalytic center, and the small domain contain-
ing ... CGM
and G
CX
+
⁄ CGM), 5-methylcytosine was introduced
into one of the strands of the recognition site instead
of the target cytosine. The melting curves of the hemi-
methyla...
... staining of 293-VnR and
ATDC5 cells expressing the Y373C mutant with anti-
FGFR3 and anti-phosphotyrosine sera showed both
intracellular and cell-surface phosphotyrosine staining
(Figs 2Eb,c and ... Conversion of
Lys650 into methionine in the TK2 domain induces
hyperphosphorylation and marked intracellular retent-
ion of the mutant receptor leading to phosphorylation
of ta...