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Báo cáo khoa học: "Enhanced expression of constitutive and inducible forms of nitric oxide synthase in autoimmune encephalomyelitis" pdf

Báo cáo khoa học:

Báo cáo khoa học: "Enhanced expression of constitutive and inducible forms of nitric oxide synthase in autoimmune encephalomyelitis" pdf

... B and C, ×132. A: TUNEL and hematoxylin, B: TUNEL and either rabbit anti-GFAP, C: TUNEL and rabbit and ED1.Enhanced expression of constitutive and inducible forms of nitric oxide synthase in ... the expression of either nNOS or eNOS increased in the brain cellsincluding neurons and astrocytes during the peak and recovery stages of EAE, while the expression of iNOS wasfound mainly in ... results showed that the three isoforms of NOS including nNOS, eNOS, and iNOS were increased in the initiation of EAE and suggested that the brain cellsincluding neurons and astrocytes are possible...
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Báo cáo khoa học: Regulated expression by PPARa and unique localization of 17b-hydroxysteroid dehydrogenase type 11 protein in mouse intestine and liver pdf

Báo cáo khoa học: Regulated expression by PPARa and unique localization of 17b-hydroxysteroid dehydrogenase type 11 protein in mouse intestine and liver pdf

... blot50CBB-stain3725IntestineLiver+ ++ +Wy14,64317β-HSD11CBB-stainBAFig. 1. Effects of Wy14 643 on protein expression levels of 17b-HSD11 in the liver and intestine of mice. (A) Specificity of ... 10’17β-HSD1117β-HSD11L-FABPL-FABPCFig. 2. Expression of 17 b-HSD11 protein in the gastrointestinaltract. (A) The gastrointestinal tract of mice [29] fed a diet containingWy-14 643 for 5 days was divided into 10 parts, and the ... levels of 17b-HSD11 in the liver and intestine. The same amounts (20 lg) of proteins from the PNS frac-tions of liver and small intestinal mucosa were analyzed by westernblotting using antibodies...
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Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx

Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx

... the findings obtained byhematoxylin and eosin staining. We examined the expres-sion of these proteins in a cancer-free layer of squamousepithelial cells, carcinoma in situ (CIS) and invasive ... Bluestaining.Table 2. Quantitation and comparison of proteins in GM and WI-38cells. From the data in Fig. 2 and others, the concentrations of Mcmproteins, ORC2 and PCNA in total protein and chromatin-bound ... shown). The intensity of the Mcm4band was clearly decreased at 22 h chase in both HeLa and WI-38 cells, indicating the presence of turnover of theprotein. Quantitation of the Mcm4 band suggested...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

... chain intoN-terminal and C-terminal centers during refolding[21]. Since then, many scientists have been interested in demonstrating this concept by using N-terminal and C-terminal fragments of ... located in the C-terminal loop of SNase playsan important role in protein stability and conforma-tional integrity. During protein unfolding ⁄ refolding,the addition of 2.5 protons (both E75 and ... Global segment interactions and the folding profiles in wild-type SNase. (A) W140, in loop 1 interacts with loop 2 and loop 3which forms a ‘lower neck’ network area in maintaining protein ter-tiary...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "What lies beneath: Semantic and syntactic analysis of manually reconstructed spontaneous speech" pdf

... types in column four of Table 1 are themost often “generated” in Pra(as they areunseen in the automatic parse Pva). Sincerules like [S → NP VP], [PP → IN NP], and [SBAR → IN S] appear in ... concep-tion of transformation (Harris, 1957). In this view,the original verbatim string is the surface form of the sentence, and as in linguistic theory should beconstrained in some manner ... be”) and others) and appear more likely than random to occur in coreargument regions of an utterance rather than in op-tional modifying material.Reconstructive insertions751Proceedings of...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "Prosodic Aids to Syntactic and Semantic Analysis of Spoken English" ppt

... the pre-processor is in two parts, one an indexed string of lexical items plus prosodic information, the other a string of tone groups indicating their start and end points: [** Ayes, 1] [**// ... two types of prosodic information, namely pitch and pause, to assist syntactic and semantic analysis during parsing. 1. INTRODUCTION In attempting to merge speech recognition and natural ... in some sense, understand the intent of a speaker in telephone-based, information seeking dialogues. As a result, we are interested in NLU to improve the semantic recognition accu- racy of...
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Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot

Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot

... establishing the memory of recombination,leading to unique nuclear organization duringmeiosis.Materials and methodsCulture of C. cinerea and collection of fruitingbodiesThe basidiomycete Coprinopsis ... diagram of the trunca-tion mutants of CcCac1L. (C) Interaction between CcCac1L and CcLim15 in a yeast two-hybrid assay. The inserts in the activation domain(AD) and DNA-binding domain (BK) ... repair[28] and in DSB repair [29,30]. Despite much accumu-lating evidence regarding the role of CAF-1 in chroma-tin assembly following various DNA synthesis events,its involvement in chromatin assembly...
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Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt

Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt

... becausesecretin-like proteins of type IV pili and type II proteintranslocation machineries are known to form ring-likestructures in outer membranes. The presence of PilQ in T. thermophilus inner and ... ª 2006 FEBSN-terminal domain of PilQ, and the colocalization of PilQ with PilW in inner and outer membranes and theresults from the pilW and pilQ mutant studies, it istempting to speculate ... compilation ª 2006 FEBSAn interesting finding was that PilA4 protein under-goes glycosylation. This is a trait of many pili proteins and has also been detected in pilin-like proteins of DNA transformation...
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

... Calometric melting curves of wild-type and mutants of SNase. DSC curves of nine proteins (WT, E73G, E75G, E101G,E122G, E129G, D77G, D83G and D95G). Lines 3 and 6 representE75G and E129G, respectively; ... (E129G) and 43.43% (E75G)lower than that of the wild-type protein, suggestingthat Glu residues at positions 75 and 129 play keyroles in maintaining the native structure of the protein.Furthermore, ... fluorescent spectra of wild-type and mutants of SNase. Spectra of nine proteins (WT, E73G, E75G, E101G,E122G, E129G, D77G, D83G and D95G). The spectrum of E129G(bold line) is much lower in intensity...
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Báo cáo khoa học: Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells pot

Báo cáo khoa học: Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells pot

... hepatic cell line HepG2, HePC hinders theincorporation of radiolabelled choline into PC, the end-product of the CDP–choline pathway. This reduction in theincorporation of choline was not due ... accumulation of radiolabel in CP and a reduction in the radioactivity associated to CDP–choline,suggesting that some of the enzymes involved in thebiosynthesis of PC must be the main target of HePC.Cytosolic ... resulted in less than 8% contamination of themicrosomes and mitochondria in the cytosolic fraction.Choline kinase assay. Choline kinase (CK) activity wasassayed by measuring the rate of [methyl-14C]cholineincorporation...
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