... Calometric melting curves of wild-type and mutants of SNase. DSC curves of nine proteins (WT, E73G, E75G, E101G,E122G, E129G, D77G, D83G and D95G). Lines 3 and 6 representE75G and E129G, respectively; ... (E129G) and 43.43% (E75G)lower than that of the wild-type protein, suggestingthat Glu residues at positions 75 and 129 play keyroles in maintaining the native structure of the protein.Furthermore, ... fluorescent spectra of wild-type and mutants of SNase. Spectra of nine proteins (WT, E73G, E75G, E101G,E122G, E129G, D77G, D83G and D95G). The spectrum of E129G(bold line) is much lower in intensity...