... (A) potential surface with fructose in the active site ‘funnel’,(B) electron density map for fructose and surrounding side-chains,(C) hydrogen bonds with numbering of residues interacting with fructose ... of the native enzymeand its complex with the product of hydrolysis – fructose.The crystals of raffinose ($ 0.1 mg) were added to the crys-tallization drop with the apo crystals of b-fructofuranosi-dase. ... the enzymebelongs to the group of hydrolases acting with reten-tion of substrate configuration. The other residuesinvolved in interactions with the fructofuranoside ringare Asn53, Gln70, Trp78,...