... 30
Time (h)
Activity (%)
0
2
4
0 5 10 15 20 25 30
Time (h)
Ln % activity
–2
0
4
8
12
16
20
70 80 90 100 110 120
Temperature (°C)
Cp (kcal/mol °C)
130
Fig. 7. (A) Kinetic stability of McoP. The activity ... Winooski,
VT, USA). Cu
+
oxidation activity was measured in terms
of oxygen consumption rates by using an oxygraph, as
previously described [5]. The optimal temperature for the
act...
... in epimerase activity (Fig. 4B) while the consti-
tutive mutarotase activity remained the same. More
importantly, with the increase in epimerase activity, the
coeluting mutarotase activity was ... epimerase activity
also showed mutarotase activity. If S. cerevisiae epimerase
were a truly bifunctional enzyme, then induction with
galactose should induce both epimerase and mutarotase...
... Prm3
+786
8
01234567
0246 12810
0 5 10 15 20 25
Luciferase Activity (RLU)
Luciferase Activity (RLU)
Luciferase Activit
y
(RLU)
Luciferase Activity (RLU)
A
CD
B
-1394
+1
Luc
+1 Luc
-975
pGL3Basic
+1
Luc
-50
+1 ... However, the level of )404 to
+119 directed luciferase activity was not significantly
different from that of the Prm3-directed luciferase
activity (e.g. )404 to +1) express...
... partial activity
could be recovered under reducing conditions, this
activity remaining threefold lower than that of the
wild-type. Figure 3 illustrates the effect of reduced
MV
+
on H–D exchange activity ... than 15%.
Proteins
Aerobiosis Anaerobiosis Anaerobiosis + MV
+
Activity
HD
formation
H
2
formation Activity
HD
formation
H
2
formation Activity
HD
formation
H
2
formation
HupU...
... structure of MGD1, only the loop
part seems to convey activity.
Activity is directed mainly against Gram-positive bacteria
and fungi
The activity spectra of peptides B, K, M and Q were
compared ... the
loop 3 sequence and its bacteriostatic activity is in
agreement with the general observation that the basicity
of defensins is an important parameter for activity, in
particular because...
... family and by modulating the intrinsic
activity (or, degree of occlusion) of glucose transporters
(Fig. 9).
Fig. 5. The involvement of tyrosine kinase activity in arsenite-induced
glucose uptake. ... Biochem. 270) Ó FEBS 2003
activity of this enzyme (in conjunction with other signals)
to induce GLUT-4 translocation in 3T3-L1 adipocytes. Of
note, the basal levels of PKC-k activity are...
... mutation resulted in the selenoenzyme Se-2F3-
scFv. The GPX activity of Se-2F3-scFv was determined by
the coupled coenzyme system. One unit of activity is defined
as the amount of compound that utilizes ... 100 m
M
potassium
phosphate buffer, pH 7.0, and an aliquot was taken for
assay of CCO activity [35]. The CCO activity was measured
in 2 mL of the reaction system, in which the cytoc...
... levels of
luciferase activity than did L4.0. Among the deletion
constructs tested, L1.2 had the highest relative luciferase
activity. L0.5 also showed a comparable high activity. These
results ... promoter activity. MKN1 cells
were transfected with wild type (K3S2) or mutated constructs. Relative
luciferase activity was determined in triplicate, and data were nor-
malized to b-galactos...