... Plasmidsharbouring the ITS1 insert in the correct orientation werelinearized by HindIII digestion, while the ITS2 insert wasfurther subcloned into the XbaI/HindIII site of the pBlue-script+ vector (Stratagene) ... processingpathway that is distinctly different from splicing ([5];unpublished data]. The other ribozyme (DiGIR1), whichalong with the I- DirI HEG is inserted in DiGIR2, carriesout hydrolysis at two internal ... I- like ribozyme DiGIR1, located within the twin-ribozyme intron Dir.S956-1. The RNA retains the majorityof Dir.S956-1 including the homing endonuclease gene and asmall spliceosomal intron, the...