... determine whether the activator-binding domains of the SWI ⁄ SNF complex are important for its recruit-ment to promoters in vivo and to determine whether the Swi1 and Snf5 domains are differentially ... some other factor. The SAGAcomplex, another Gal4-associated co-activator[30,47,48], is a potential candidate for such a factorbecause partial redundancy between SWI ⁄ SNF and Gcn5, the histone ... ng of total RNA per reac-tion, and expression of ARN1 was used for normalization. For two-step qRT-PCR, cDNA was prepared using iScriptcDNA Synthesis kit (Bio-Rad), containing oligo-dT and random...