... respectively. Km and Vmax for NAD and NADH of Pos5p have not been reported [2,3].Constructions of double and triple mutants for UTR1, YEF1 and POS5To examine the roles of Yef1p, Utr1p and Pos5p, ... regulation of Yef1p and Utr1p by NADPH, NADH and NADP (Table 2) [1], and the different transcriptional patterns and protein–protein interactions of Yef1p, Utr1p and Pos5p [25–27] may be indicative of ... 2005 FEBS 3345 and that of Utr1p was 3.9 mm; this was similar to and higher than the Kmvalue of the NAD of Yef1p(1.9 mm) and Utr1p (0.5 mm) [1], respectively. Vmax for the NADH of Yef1p was...