... Inc.inthefreeform,andconsequentlyavailableforrapiduptake,remainsunknown.Thisadsorptionandtheconcurrentloweravailabilityforbacterialuptakemightcauseanunder-estimationoftheactualbacterialproductiononandinpolysaccharide-richmaterialsuchasmarinesnow(44),relativetobacterialenzymeactivity.ThecouplingbetweenhydrolysisanduptakeofDOMinparticle-associatedandfreebacteriaisstillnotfullyunderstood.Thereasonswhytheattachedbacteriabenefitsolittlefromtheirstronghydrolyticactivities,iftherearenolimitingfactorsinterferingwiththeuptakeofenzymatichydrolysisproducts,areunknown.Thisfundamentaldiscrepancyshouldbemorethoroughlyinvestigatedinordertoimproveunderstandingofthebiogeo-chemicalfluxoforganicmatterandtheroleofbacteriainthecyclingofDOMintheocean.Inanycase,itiswellacceptedthatparticledecomposition(45)contributessignificantlytothelossoforganicmaterialfromsettlingparticlesduringsinkingandthusdeterminestheefficiencyofthebiologicalCpump(organicmattertransportfromtheseasurfacetotheseabed).D.EnvironmentalFactorsInfluencingEnzymaticActivityThemagnitudeofthemainextracellularenzymeactivitiesinmarinewaterisfrequentlyintheorderaminopeptidaseϾphosphataseϾβ-glucosidaseϾchitobiaseϾesteraseϾα-glucosidase.However,exceptionsmayoccur,asobservedbyChristianandKarl(46)intheequatorialPacific,whereβ-glucosidasewasaboutfourtimeshigherthanaminopep-tidase.Thissuggeststhattheremaybefactorsregulatingactivitiesonalargescale.How-ever,knowledgeofglobalregulatingfactorsisscarce.ChristianandKarl(47)foundthathistidineandphenylalanineinhibitedaminopeptidaseexpressioninAntarcticwaters.Like-wise,KimandLipscomb(48)suggestedthatmetalsmayberegulatingfactorsforproteases(leucineaminopeptidaseseemstobeprincipallyaZn2ϩ-dependentenzyme).ThiswasespeciallyduetoZn2ϩ(whichisrareinmarinewaters),butMn2ϩ,Co2ϩ,Fe2ϩ,andMg2ϩmightalsoplayarole(47–50).Inthesurfacelayeroftheocean,ultraviolet-Bradiationcanbeimportant,mainlythroughphotochemicaldegradationoftheextracellularenzymes(51,52).Withrespecttophosphataseactivity,theabundanceofinorganicPisregardedasaregulatingfactor,particularlyfortheP-limitedregionsintheoceans(53–55).However,dissolvedorganicphosphorus(DOP)andparticulateorganicPalsoshouldbeconsidered(56).Furthermore,mechanismsofphosphataseregulationaredifferentforbacteriaandphytoplankton.WhilethephosphatasesofphytoplanktonseemtoberegulatedstrictlybyinorganicPconcentrations(49,57–59),thismechanismisnotsoclearforbacterialphosphatases.ThelattermaytargetCandNratherthanPsupply,aspointedoutforthelimneticenvironmentbySiudaandGu¨de(60)andforthedeepandC-limited,butphos-phate-replete,oceanbyHoppeandUllrich(61).Inanycase,regardlessofenvironmentalfactors,variationofspeciescompositionwithinthebacterialcommunitycansignificantlyinfluencethedistributionofenzymeactivitiesinthesea(62,63).Theeffectsofenvironmentalfactorsonenzymeregulationarereflectedbythediver-sityofextracellularenzymes,asexpressedinthepossiblerangesofKmandthepatternsofindividualcell-specificenzymepotentials(Table2,Table3).InformationontheKmvalues ... zone between land and the open sea. Thus theyare frequently characterized by local morphological and hydrographical patterns and bygradients of salinity, eutrophication, pollution, and sediment ... Contribution of Different N Sources to Bacterial N DemandDFAA DCAA NHϩ4 Environment Conditions (% of N demand) (% of N demand) (% of N demand) Experiment ReferenceDelaware Bay estuary Exponential...