... 5’ biotin tag used for mobilization.•The beads are magnetized and attract the biotin in the B adaptors.Introduction*•Read lengths are around 200-300 bases.•400,000 reads of parallel sequencing•100mb ... nucleotide bases are added in a timed fashion (beginning with A, T, G, C with 10s between each nucleotide and a successive apyrase wash, followed by the next nucleotide.)•As a bi-product of incorporation, ... Mess•There are four adaptor combinations that are formed from the ligation.•A sequence A•A sequence B•B sequence A•B sequence BPost emPCR•The micro-reactors are broken, and the beads are...