... a final concentration of 0.3% (v/v) and incubated at 37oC for 24 h with shaking. Sterility testing was performed by placing 0.5 ml of the killed vaccine into 25 ml of enrichment broth, incubated ... were vaccinated subcutaneously (SC) in the neck twice with 5 ml of the inactivated multivalent whole cell vaccine at 6 weeks and then again at 2 weeks before parturition. The two remaining gilts, ... be involved in adherence to and invasion of cells in culture and is related to Ail in Yersinia, and pagC in Salmonella. As I am not fully convinced, we will run another SDS-PAGE and this time,...