... 105)werewashedwithphosphate-buffered saline (PBS; Sigma-Aldrich) and re-suspended in 50 μl of staining solution (PBS, 3% FBS and 0,1% NaN3) containing an optimal concentration of antibody. After a 20-minute incubation in ice, ... e institutional ethical review board of the Istituto Oncologico Veneto, in accordance with the ethi-cal standards of Helsinki Declaration.Cyclosporin A (CsA, Sandoz Pharmaceuticals AG;Cham, ... Natl Acad Sci USA 2009,106:17469-17474.9. Kaneko S, Mastaglio S, Bondanza A, Ponzoni M, Sanvito F, Aldrighetti L,Radrizzani M, La Seta-Catamancio S, Provasi E, Mondino A, Nagasawa T,Fleischhauer...