... were obtained from Qiagen(Valencia, California, USA). The siRNA target sequenceswere as follows: CHK1 -A, AAGAAAGAGATCTGTATCAAT;CHK1-B, TTGGAATAACTCCACGGGATA; CHK1-C,AACTGAAGAAGCAGTCGCAAGT; CHK1-D, ... ACGCCGTCCTTT-GAATAACAA; CHK2-B, AGGACTGTCTTATAAAGATTA;CHK2-C, CAGGATGGATTTGCCAATCTT; and CHK2-D,CTCCGTGGTTTGAACACGAAA. The sequences used in HT-RNAi screening were the A and B sequences for bothCHK1 and ... siRNA. In addition, we functionally-validated the combination of gemcitabine and CHK1 inhibitors as a potential treatment for pancreatic cancer patients. The preclinical finding of inhibition of...