... stand-
ardized a one step single tube protocol for rapid
serotyping of dengue viruses. This assay can be performed
rapidly with in a period of 4 hours compared to 8 hours
in two -step typing assays. ... 3' and Ts4:
5'TGTTGTCTTAAACAAGAGAGGTC3'), as reported earlier
[6].
Single -step Dengue multiplex RT-PCR (M -RT-PCR)
A one- step single tub...
... Division of Infectious Diseases, Brown University, Providence, Rhode Island,
2
Assistant Professor,
Division of Infectious Diseases, Stanford University, Stanford, California and
3
Professor, ... Division of Infectious Diseases, Stanford University,
Stanford, California
* Corresponding author
HIV Diversity and Drug Resistance
HIV-1 group M, the major pathogen responsible for...
... if one of the following two
conditions holds: (i) their nearest ancestor has the distance of
exactly x hops to one of them and the distance of at most x
hops to the other one, or (ii) one of ... 4–11) of the five free codes as 1, 3,
3, 3, and 3, respectively. CBP computes the W
2,j
values of
codes C(3, 2) to C(3,5)as1,1,2 ,and2 ,respectively ,and
then chooses one o...
... FS ∩ FT for the set of all common fixed points of the mappings S and T.
Lemma 2.1. Let C be a nonempty closed convex subset of a normed space E.LetS, T : C → C be
asymptotically nonexpansive ... that one needs two different sequences {s
n
} and {t
n
}
for the mappings S and T used in 1.3, but it is readily answered when one takes k
n
sup{s
n
,t
n
}. Henceforth, we wi...
... dementia and the development of caregiver
stress and burden [5]. Families often report that behavio-
ral disturbances are the primary source of stress and the
primary cause for institutionalization of ... York: Oxford University Press;
2005.
BioMed Central
Page 1 of 12
(page number not for citation purposes)
Health and Quality of Life Outcomes
Open Access
Research
De...
... measure for child
and adolescent acceptance of asthma, to measure one
aspect of quality of life believed to influence one& apos;s overall
quality of life. Adolescents with asthma identified level of
participation ... important considera-
tions in development of the scale:
1. Level of participation in self-selected activities offers a
measure of one aspect of quali...
... medi-
cal professions.
As a point of criticism, using this methodology is very
time and effort intensive; observational data contains an
extremely wide amount of information. The more infor-
mation ... reorganization of categories and addi-
tions or deletions of tasks. After all task lists were verified
for completeness and accuracy they were implemented in
the data collection...
... from peak) had a sensitivity of 67% for a viral load of >1000 copies/mL, a specificity of 82%, and
identified 22% of patients for viral load testing. Sensitivity of the WHO-based algorithm was ... Frederick, MD, USA,
4
National Institute of Allergy and Infectious Diseases, National Institutes of Health, USA and
5
Institute of Tropical
Medicine and University...
... studies
related to assay development, Khetemenee Lam and Stephane Olland for pro-
viding rhIL21, and Mary Collins, Cheryl Nutter and Davinder Gill for critical
review of the manuscript.
Author ... contributed to the writing of
the manuscript. MFS was responsible for interface with the clinical team and
for securing and scheduling the resources that will be required up...
... for 30 sec, 59°C for
45 sec and 72°C for 60 sec, and finally 72°C for 5 min.
The PCR reactions were carried out in 25 μlvolumesin
thepresenceof6ng/μl of each of the forward and the
reverse primers ... PCR and ISH results show that 62 out of 67 (92.5%)
and 64 out of 67 (95.5%) positively expressed varying
levels of IGF2-P4 and H19, respectively.
Comparison of th...