báo cáo hóa học: " Inhibition of gap junctional Intercellular communication in WB-F344 rat liver epithelial cells by triphenyltin chloride through MAPK and PI3-kinase pathways" pdf
... al., Inhibition of gap junctional Intercellular com-
munication in WB-F344 rat liver epithelial cells by triphenyltin chloride
through MAPK and PI3-kinase pathways Journal of Occupational Medicine ... Cytotoxicity of TPTC in WB-F344 liver cell. (A) Cell viability of WB-F344 liver cells after exposure to TPTC of different concentrations f...
... area) and are nestin and CD34 positive, and
keratin 15 negative [72-75]. The mouse hfPS cells were
impl anted into the gap region of the severed sciatic and
tibial nerve o f mice. These cells, ... presence
of a growth factors delivery cell system capable of
responding to stimuli of the local environment during
axonal regeneration.
In the present study, we aimed at inves...
... state. In NTera2 cells, differen-
tiation status is confirmed by decreases in expression of
Oct4 and Nanog and increases in expression of Afp, Ncam1
and Eno3. 2102Ep cells alter expression of these ... differentiation of
NTera2 cells. In contrast, the level of differential expression
of each gene is within 1.0 fold in both undifferentiated and
differenti...
... examined by using selective a NFκB inhibitor and
measuring IκBα protein levels by western blots. A role for IL-1β in NOS2 induction was tested by examining
effects of caspase-1 inhibitors and using ... levels in the incubation
media were assessed by ELISA; and (B) NOS2 activity in the cells measured by increase in net DCF fluorescence (total RFU
minus the RFU obt...
... 70C), in a rat model of focal cerebral
IR. BW-B 70C demonstrated a neuroprotective role
through inhibition of both 5-LOX and NF-κB. It is a
potent and a selective inhibitor of 5-LOX in vitro and in
vivo ... observations indicate that 5-LOX protein
is up regulated mainly in microglia/macrophage and neu-
rons in the ipsilateral hemisphere after IR injury.
Inhibiti...
... the
addition of the substrate solution to the sPLA2 containing
samples. Kinetic parameters including the properties of
CHEC-9 were determined by measuring the initial veloci-
ties (Vo) of enzyme ... substrate was complex. The expected progression
of activity with increasing substrate concentration was
often interrupted abruptly at high substrate concentra-
tions (> 25 μM) by pr...
... staining of brain tissue sec-
tion using an anti-CD11b Ab revealed infiltration of
CD11b-positive inflammatory cells at the contusion site
of brain-injured mice in the placebo group, but not in ... 1:20 (in 0.1%
milk in PBS containing 0.05% TWEEN) and murine
recombinant mouse C5a at defined concentrations for
establishing the standard curve. After incubation and sub-
sequen...
... report
Inhibition of foot -and- mouth disease virus replication in vitro and in
vivo by small interfering RNA
Wang Pengyan, Ren Yan, Guo Zhiru* and Chen Chuangfu*
Address: College of Animal Science and ... machinery would be to defend cells
against viruses that express dsRNA as part of their life cycle
[4]. Indeed, there is compelling evidence indicating that
RNAi is cr...
... of epithelial cells
by multiple mechanisms.
Methods
Virus and cells
The human Long strain of RSV (ATCC, Manassas, VA,
USA) was propagated in monolayers of Hep-2 cells grown
in Eagle's minimum ... at moi 3, and
then INF-a was added into culture at the indicated time points at a final concentration of 1000 U/mL for 30 min. Un-infected
cells were treated with IF...
... mediated by infiltrating immune cells.
Taken together, our data demonstrate that SARS-CoV in
general is a weak inducer of cytokines and antiviral genes
in non-lymphatic cells. Chemokines like IP-10 and ... as of the interferon-
induced antiviral genes ISG56 and MxA, the chemokine RANTES and the interleukine IL-6.
Interestingly, however, SARS-CoV strongly induced the chemo...