... Department of Medical Pharmacology, Università di Milano, Milan, ItalyFull list of author information is available at the end of the articleRadaelli et al. Journal of Translational Medicine ... and p105Rb, leading to transfor-mation and carcinogenesis [2], facilitate cell immortalisa-tion in primary human keratinocytes [3], increasegenomic instability [4], and maintain the transformedphenotype ... 55°C, and washed again. The amplifier reagent was then added, and the samplesincubated for 1 h at 55°C; after further washing, this wasreplaced by the label probe reagent for 1 h at 50°C. Afterwashing,...