... acarbon-coated formvar grid, left for 1 min, fixed with glu-taraldehye, wicked dry with filter paper, and 2% uranylacetate was added and the mixture was incubated for 2 min. The grid was wicked dry and ... buffer supplied with the enzyme, and contained Aba, Abb and Abcat40 nm each, and the start and stop primers Abstart and Abstop at 600 nm each, and 200 lm each of dATP, dCTP,dGTP and dTTP. The ... was cut out from the gel, purified using a GFX PCR and gel band purification kit (GE Healthcare, ChalfontSt Giles, UK). The gene was digested with NdeI and SacIrestriction enzymes and subjected...