... of C1 inhibitor and membrane cofactor protein mRNAsMasato Hosokawa, Andis Klegeris and Patrick L McGeer*Address: Kinsmen Laboratory of Neurological Research, University of British Columbia, ... Burlington, ON, Canada), and incubated at37°C for 20 min. Subsequently DNase I (from bovinepancreas, Pharmacia Biotech, Baie d'Urfé, PQ, Canada)was added to reach a final concentration of ... theirmRNAs were extracted. Immunostaining with antibodiesagainst CD68 (Dako, Mississauga, ON, Canada) whichstains microglia as well as macrophages, and glial fibril-lary acidic protein (GFAP, Dako),...