Báo cáo sinh học: " Development of an improved microneutralization assay for respiratory syncytial virus by automated plaque counting using imaging analysis" pdf
Báo cáo sinh học: " Development of an improved microneutralization assay for respiratory syncytial virus by automated plaque counting using imaging analysis" pdf
... contributionsDesign and conception of the study and co-drafted themanuscript (DPY); development of the methods and co-drafted the manuscript (EZ); assisted in the development of the automated plaque counting ... agreement andequivalence between traditional manual and automated plaque counting methods for detection of RSV neutraliz-ing antibody titers. The 180 tests performed in the pres-ence and absence of ... presence and absence of complementand sorted the data by assay, method, and complementtreatment. The range and mean of the replicate tests aredepicted in Fig. 3. The difference in the mean of 136...
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báo cáo hóa học:" Development of an improved microneutralization assay for respiratory syncytial virus by automated plaque counting using imaging analysis" pptx
... CentralPage 1 of 5(page number not for citation purposes)Virology JournalOpen AccessMethodology Development of an improved microneutralization assay for respiratory syncytial virus by automated plaque ... agreement andequivalence between traditional manual and automated plaque counting methods for detection of RSV neutraliz-ing antibody titers. The 180 tests performed in the pres-ence and absence of ... presence and absence of complementand sorted the data by assay, method, and complementtreatment. The range and mean of the replicate tests aredepicted in Fig. 3. The difference in the mean of 136...
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Báo cáo sinh học: " Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity" pptx
... responsible for cleavage of the A17 membrane protein, at an Ala-Gly-Ala site [9].This consensus Ala-Gly-Xaa cleavage site of vaccinia issimilar to that used for both the adenovirus and Africanswine ... Chelsea M Byrd - cbyrd@sgph.com; Dennis E Hruby* - dhruby@sgph.com* Corresponding author AbstractThrough the use of transient expression assays and directed genetics, the vaccinia virus (VV) ... Honeychurch for constructing pTM:L4R, pTM:A3L, and pTM:A10L, Rich Condit for providing ts16, and TransTech Pharma for supplying TTP-6171, TTP-1021, and TTP-0961. This work was funded by NIH grant...
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báo cáo sinh học:" Development of a quality assurance handbook to improve educational courses in Africa" docx
... the idea of the handbook and managed theproject. HN conducted a detailed review of the literatureand produced the first drafts of the handbook and thispaper. SP provided case studies and technical ... quality of courses and ensurethat courses remain relevant in the light of developingknowledge in the discipline.We stress the importance of having clear procedures for approval of new courses and ... accordance with their local resource capacity. Wesubsequently piloted this handbook at one of the sites in Ghana. The feedback from this aided the development of the handbook. The development of...
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Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
... (Dept. of Agriculture and Food, Republic of Ireland). Thank you to Mr. Noel Shanaghy and Mrs. Breda Doody of Waterford Regional Hospital, Mr. Eddie Beggan of Limerick Regional Hospital and Dr. ... slope of -3.5 and -3.7 respectively for GI and GII NoV (Fig. 1B and Fig. 2B). Melting curveanalysis showed a positive peak at ~90°C and 88°C for GIand GII NoV. The broad reactivity of the assay ... assumption of 100% RT efficiency. This method allows a calculation of the minimum amount of NoV present based on cDNAvalues. Ideally quantification of an RNA virus involves theuse of an RNA standard....
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Báo cáo sinh học: " Development of a model for marburgvirus based on severe-combined immunodeficiency mice" doc
... for analysis of therapeutics and candidateantibodies. It will be much more efficient for the purposes of quickly screening lead compounds and neutralizingantibodies than guinea pigs or nonhuman ... days postinfection showing presence of Marburg viral antigen (brown). Magnifications were 4× for panels A, C, and E, 20× for panels B, D, and F, and 40× for panels G-H.Virology Journal 2007, ... Pre-and postexposure prophylaxis of Ebola virus infection in an animal model by passive transfer of a neutralizing humanantibody. J Virol 2002, 76(12):6408-6412.25. Bray M: The role of the...
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Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx
... randomassortment of thirty macaques housed at the WaNPRCand analyzed using the standard RV2 and OSM QPCRassays. While all of the samples were positive for the singlecopy OSM gene, only six of the thirty ... ContributionDesign and conception of the study (AGB, TMR); develop-ment of the methods for amplification of the ORF59/60regions (AGB, TMR); Development of the QPCR assaysand quantitative analysis (AGB, ... withinmacaque RV1 rhadinoviruses. In this paper, we report the development of a sensitive and specific TaqMan QPCR assay and its use in detecting and quantitating RV2 rhadi-noviruses from different...
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báo cáo sinh học:" Training of front-line health workers for tuberculosis control: Lessons from Nigeria and Kyrgyzstan" docx
... usuallyuneven. Since failure of control measures is an importantdeterminant of the distribution and spread of tuberculosis[29], it is important to focus trained human resources inareas of high tuberculosis ... detection.The urban slums of Moscow are populated by poormigrant workers from Kyrgyzstan and other Central Asiancountries, many of whom contract tuberculosis due tocongested living conditions and limited ... is an important component [2].DiscussionMany factors encumber the evaluation of the contribution of training to tuberculosis health workers' performance. For example, sub-optimal human...
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Báo cáo sinh học: " Role of HIV-1 subtype C envelope V3 to V5 regions in viral entry, coreceptor utilization and replication efficiency in primary T-lymphocytes and monocyte-derived macrophages" docx
... insertion of theV3 region of an R5 isolate into an X4 molecular clonealtered the tropism of an X4 isolate to an R5 phenotype[10,19,23,28-30].Our in-silico analysis of the V3 sequences of the ... India. We thank AIDS Reference and Reagent Program for providing HIV-1 isolates and cell lines and Roshni Mehta and Brian Wellensiek of Ahmad Lab for critically reviewing this manuscript.References1. ... X, Huang Y, Gettie A, Ba L, Blanchard J, Ho DD: CD4+lymphocytopenia in acute infection of Asian macaques by avaginally transmissible subtype-C, CCR5-tropic Simian/Human Immunodeficiency Virus...
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Báo cáo sinh học: " Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats" potx
... investigation conformed to the Principles of Laboratory Animal Care formulated by the NationalSociety for Medical Research and the Guide for theCare and Use of Laboratory Animals published by theU.S. ... on a scale of 0-18 (normal score 0,maximal deficit score 18)Statistical analysisData were presented as mean values and standard devia-tion. A method of ANOVA (analysis of variance) withScheffe’s ... Ministry of Education,Fujian Normal University, for their technical assistance. This work wassupported in part by the Natural Science Foundation of Fujian Province of China (2008J0282) and by the...
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