... domain,
the overall layout of chorismate and prephenate processing
sites in the T-protein resembled that of the P-protein.
However, results from the present study showed that
the organization of the ... prephenate
dehydrogenase.
The final step in the biosynthesis of tyrosine in Escherichia
coli and other enteric bacteria is the transamination of
p-hydroxyphenylpyruvate, whi...
... of
X-rays. This term defines the level of detail, or the
minimum distance between structural features that can
be distinguished in the electron-density maps. The
higher the resolution, that is, the smaller ... part of the protein is rather doubtful. (e) The distances between the ion and the coordi-
nating atoms are shown with four decimal digit precision, vastly exceedin...
... supports this
conclusion. The other antibodies in this assay did not
hinder the interaction of TC with Cbl at all (Table 3)
and therefore we cannot draw any conclusions on the
involvement of the other ... transcobalamin.
The N- and C-termini are indicated by the
corresponding letters (N-terminus is hidden
behind the a helix). The colors of different
regions correspond to th...
... noncovalently with the active site of the
proteinase [1]. In the locking step that follows, the P
1
–
P
1
¢ bond is cleaved [2,3] and the P
1
residue is coupled to
the active site serine of the proteinase ... Relative to (A) the structure shown in (B) is turned approximately 180° around the y-axis and
approximately 45° around the x-axis of the coordinate system sho...
... protease-resistant isoform of the
prion protein in brains of individuals with these diseases.
This abnormal form of the protein (PrP
Sc
) is also suggested
to be the infectious agent in the disease on the ... PrP
c
could be in the
femtomolar range [18]. Despite these inconsistencies, the
conclusion that PrP
c
is a Cu-binding protein is widely
accepted. The implication of...
... throughout the
body. Their expressions are tissue-specific, but in disease states such as cys-
tic fibrosis, inflammation and cancer, this specificity can be disturbed.
MUC5AC is normally expressed in the ... loaded is shown by the
reactivity of the mAb against myc. One reason for the
requirement of the N-terminal 91 amino acids could
be that these are necessary for the correct...
... were
positioned within the catalytic domain of the enzyme.
The role of the particular oxygen atoms of the selected
phosphate groups is also discussed. Moreover, we ana-
lyzed the function of the oxygen moiety ... in the concentration
of metal ions present in the catalysis reaction, and the
k
Mn
obs
value for this mutant enzyme was three-fold
higher than the k
Mn
obs
va...
... interaction. In all plots, the arrow on the left indicates the beginning of the associ-
ation phase; the beginning of the dissociation phase is marked by the arrow on the right.
Interaction site ... (HBS).
Discussion
In cyanobacteria, it has previously been shown that
the disulfide bridge on the N-terminal part of CP12
is involved in binding PRK, whereas the C-termina...
... a role
in the enzyme’s mechanism of action. The data pre-
sented here show that the glutarate part of inhibitor is
essential for the affinity to the GCPII, whereas the
S1 pocket of the enzyme ... such that the orientation and
binding of the glutamate residue is identical to the crystal
structure of the rhGCPII ⁄ glutamate complex, and the
N-acetyl-l-aspartate part o...
... recognized by the
activated a
IIb
b
3
integrin. Therefore, in the context of this
study, the fine mapping of the fibrinogen-binding domains
on the a
IIb
subunit was accomplished and their potential
role ... peptide synthesis (SPPS) [29–
31]. A spare glycine was incorporated as the C-terminal
residue (shown in parenthesis in the peptide sequences
below) to simplify and reduce th...