... advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the relationship between protein stabilization by osmolytes and its con-sequent ... [21–25], and DGD° values in the presence of these osmolytes are given in Table 1. How-ever, DGD° values of RNase-A in the presence of alanine, serine, lysine, b-alanine, taurine and dimethyl-glycine ... hydration of the protein domain is the driving force of protein stabiliza-tion or folding [3,4]. Furthermore, the effect of osmolytes on the functional activity of an enzyme hasalso been investigated...