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báo cáo hóa học: " Feasibility and acceptance of electronic quality of life assessment in general practice: an implementation study" pdf

Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

... ferredoxins in vivo is of particularimportance for the improvement of this coupled assay and also with respect to obtaining a deeper under-standing of the metabolism of H. thermophilus. Indeed,this ... syn-thesis in vivo.To investigate the reaction mechanism of H. thermo-philus POR, we measured the EPR spectra of theenzyme in the presence of various combinations of sub-strates. Intra- and intermolecular ... address*Research Institute for Nanodevice and BioSystems, Hiroshima University, JapanInstitute of Biogeoscience, Japan Agencyfor Marine-Earth Science and Technology(JAMSTEC), Kanagawa, Japan(Received...
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Tài liệu Báo cáo khoa học: Pathways and products for the metabolism of vitamin D3 by cytochrome P450scc docx

Tài liệu Báo cáo khoa học: Pathways and products for the metabolism of vitamin D3 by cytochrome P450scc docx

... form of vitamin D3, 1a,25-dihydroxyvitamin D3, displays anti-cancer, immunoregulatory and endocrine effects, and is also a potent antiproliferative and differentiation-inducing agent in different ... monohydroxyvi-tamin D3.Because cyclodextrin provides an artificial means of holding vitamin D3 in solution, we also examined themetabolism of vitamin D3 incorporated into themembrane of phospholipid ... 8. Identification of minor metabolites of vitamin D3. Products of P450scc action on vitamin D3 with retention times of 32, 26 and 26.7 min in Fig. 1 were purified, incorporated into phospholipid...
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Tài liệu Báo cáo khoa học: Diversity and junction residues as hotspots of binding energy in an antibody neutralizing the dengue virus doc

Tài liệu Báo cáo khoa học: Diversity and junction residues as hotspots of binding energy in an antibody neutralizing the dengue virus doc

... alanine scanning of theL-CDR3 and H-CDR3 loops of antibody mAb4E11.This scanning allowed us to identify the residues of these loops that contributed to the energetics and kinet-ics of the interaction ... numbering of the amino-acid sequences of immunoglobulins and the definition of their CDR loops, as described from 1997 [13]. The number-ing and definition of the L-CDR3 and H-CDR3 loops of mAb4E11 ... the kinetics of interaction between theFab4E11-H6 fragment and its MalE-E3-H6 antigen,we measured the corresponding rate constants, kon and koff, for the wild-type and mutant derivatives of Fab4E11-H6....
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Báo cáo khoa học: Flexibility and communication within the structure of the Mycobacterium smegmatis methionyl-tRNA synthetase Henrik Ingvarsson and Torsten Unge potx

Báo cáo khoa học: Flexibility and communication within the structure of the Mycobacterium smegmatis methionyl-tRNA synthetase Henrik Ingvarsson and Torsten Unge potx

... theKMSKS domain in yellow and the anticodon domain in blue. Theintervening helices a2 and a8 in the catalytic domain are coloredcyan. The linking p-helix a13 between the KMSKS domain and theanticodon ... catalytic domain, theN-terminal end of strand b4 and helix a5, and theC-terminal end of strand b8 belonging to the CPdomain (Figs 1 and 2). Each MetRS binds one phos-phate molecule coordinated by ... thecatalytic domain and to the CP domain.Residues in cyan belong to the anticodondomain of a symmetry-related protein. Theinteractions holding the CAPS in its placeare mainly of a close-packing type....
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Báo cáo khoa học:

Báo cáo khoa học: "Exploring and Exploiting the Limited Utility of Captions in Recognizing Intention in Information Graphics∗" pdf

... RecognizingIntention in Information Graphics∗Stephanie Elzer1 and Sandra Carberry2 and Daniel Chester2 and Seniz Demir2 and Nancy Green3 and Ingrid Zukerman4 and Keith Trnka21Dept. of ... Durfee, and M. Wellman. 1994. The auto-mated mapping of plans for plan recognition. In Proc. of Uncertainty in AI, 344–351.S. Kerpedjiev and S. Roth. 2000. Mapping communica-tive goals into conceptual ... inves-tigate the problem of understanding the intendedmessage of an information graphic. This paperhas focused on the communicative evidence present in an information graphic and how it can...
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Báo cáo khoa học: Solution and membrane-bound chaperone activity of the diphtheria toxin translocation domain towards the catalytic domain

Báo cáo khoa học: Solution and membrane-bound chaperone activity of the diphtheria toxin translocation domain towards the catalytic domain" doc

... conformations, in solution,membrane-bound, and membrane-inserted, stabilizespartially unfolded states of the C domain. In doing so,the T domain favors membrane binding and mem-brane penetration of the ... the A chain, correspondingto the catalytic (C) domain, and the B chain, corre-sponding to the translocation (T) and receptor-bindingdomains. The C and T domains remain covalentlylinked by ... lipidbilayer.ResultsRecombinant proteinsFive recombinant proteins were used in this study: C and T, corresponding to the isolated C and T domains,CT, corresponding to a truncated diphtheria toxinlacking the R domain,...
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Báo cáo khoa học: Structure and potential C-terminal dimerization of a recombinant mutant of surfactant-associated protein C in chloroform/methanol ppt

Báo cáo khoa học: Structure and potential C-terminal dimerization of a recombinant mutant of surfactant-associated protein C in chloroform/methanol ppt

... Go¨ttingen, GermanyThe solution structure of a recombinant mutant [rSP-C (FFI)] of the human surfactant-associated protein C(hSP-C) in a mixture of chloroform and methanol wasdetermined by ... bystandard procedures for small proteins [13] using thecomputer programNDEE(Spin Up, Lu¨nen, Germany).Owing to the high abundance of the amino acids valine,leucine and isoleucine in the ... 271) Ó FEBS 2004Structure and potential C-terminal dimerization of a recombinantmutant of surfactant-associated protein C in chloroform/methanolBurkhard Luy1, Alexander Diener2, Rolf-Peter...
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Báo cáo Y học: Production and chemiluminescent free radical reactions of glyoxal in lipid peroxidation of linoleic acid by the ligninolytic enzyme, manganese peroxidase pot

Báo cáo Y học: Production and chemiluminescent free radical reactions of glyoxal in lipid peroxidation of linoleic acid by the ligninolytic enzyme, manganese peroxidase pot

... (SFAs) and unsaturated fatty acids(USFAs) including linoleic acid at an incipient stage of cultivation and consume them with concomitant formation of lipid hydroperoxide and TBARS during prolongedcultivation ... monitoring the initial rate of increase in absorbance at 470 nm in the presence and absence of manganese. One unit of enzyme activity isdefined as the amount of enzyme that oxidizes 1 mmol of 2,6-DMP ... EI/GC/MS analysis of the reaction products and authentic 19aldehydes and seven ketones demonstrated that glyoxal,1-hexanal and 1-pentanal were formed from linoleic acidby MnP in any of the buffer...
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Báo cáo khoa học:

Báo cáo khoa học: "NLP and the humanities: the revival of an old liaison" potx

... nature and role of metadata in the hu-manities. In the next section the role of metadata in the humanities and the the ways in which theycan facilitate and enhance the application of text and ... concept of mining. By focussing on the integration of meta-data in the models underlying the mining tools and searching for ways to increase the involvement of metadata generators, both experts and ... digi-10use of annotation tools and the analysis of query logs.Protocols and tools for the mark-up of content,the specification of links between collections,the handling of IPR and privacy...
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Báo cáo khoa học: CpG and LPS can interfere negatively with prion clearance in macrophage and microglial cells pdf

Báo cáo khoa học: CpG and LPS can interfere negatively with prion clearance in macrophage and microglial cells pdf

... proceduresReagentsPK and Pefabloc proteinase inhibitor were obtained fromRoche, Mannheim, Germany. LPS from Escherichia coliwas obtained from Sigma, Deisenhofen, Germany. CpG and GpC motif-containing oligodeoxynucleotides ... phagocytosis.DiscussionThe aim of our study was to investigate the impact of the stimulation of macrophages and microglial cellsby LPS or CpG on PrPcexpression and their han-dling of prion-infected brain material. ... cells (lane 6) and, after 48 h, also in CpG-ODN-treated cells (lane 11) was increased ( 1.8- and 1.3-fold, respectively) relative to the baseline signaldirectly after infection (lanes 2 and 3)....
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