... around which the short a-heli-cal LID domain, situated in the C-terminal moiety,and the NMP-binding (NMPbind) domain move in aninduced-fit mechanism, closing upon binding of the phosphate ... further explore the contribution of theseamino acids interacting with the pyrimidine ring to the catalysis of E. coli CMP kinase. Substitution of the side chain from a well-structured protein ... the loss of the self energy in the wild-type enzyme of the residue that was mutated,DGpairwisei;jðWTÞ are the pair wise energies of the original resi-due ‘i’ in the native protein with the...