... selection of
multiple nonsynonymous mutations in tat in a unique
epidemiologically-linked cohort following transmission
of HIV-1. Comparisons of the relative transactivation abil-
ities of the Tat ... and R52 participates in the
binding of Tat to TAR and is involved in the nuclear local-
isation of Tat [18,19]. The strong or total suppression of...
... Access
Short report
Functional relevance of nonsynonymous mutations in the HIV-1 tat
gene within an epidemiologically-linked transmission cohort
Haran Sivakumaran
1,2
, Bin Wang
3
, M John Gill
4
, ... little in known about selection of mutations in the
HIV-1 regulatory genes. One of the major regulatory genes
of HIV-1 is tat, which encodes th...
... also elevated in
many of the MDSC-inducing cell lines, particularly ovar-
ian and cervical cancer cell lines, and PGE
2
in
combination with GM-CSF induced weak suppressive
function in CD33
+
cells([16],datanotshown).How-
ever, ... function . These
data and methods provide an important pre-clinical tool
for other investigators to examine other aspects of
human MDSC biology and t...
... distinct from that exhibited
by the other IE mutants in that the VP16 gene residing in
the superinfecting n212 genome was efficiently expressed
while the corresponding gene of KM110-R remained
silent. ... by the
superinfecting virus. These data suggest that the inhibitory
mechanism renders many (if not all) of the cis-acting ele-
ments (eg. promoters and origins of...
... also
contains a GAL4 gene under the control of the GAL10 pro-
moter. The induction of the yeast, by galactose containing
media, results in overexpression of GAL4, in turn inducing
an increase of the ... suggests the authenticity of its ligand
binding and the maintenance of the coding ability at
the receptor level. Consequently this suggests that gly-
cosy...
... residues W101 and W162 are
involved in the b inding of the UDP-galactose donor, t he
residue W315 in the binding of the N-acetylglucosamine-b-p-
nitrophenol acceptor, and the domain including E264
appears ... found in
b1,3-GalTs and b1,3-N-acetylglucosaminyltransferases,
thereby suggesting that they are more likely involved in
mediating the b1,3-linkage speci®city r at...
... manage,
perform four generic tasks. These include planning, organ-
izing, leading and controlling. Planning involves defining
goals and mapping out ways to reach them; organizing
entails arranging ... audience of clinicians, educationists and
health management experts, defined a list of health man-
agement competencies and performance indicators to
assist in the development of train...
... has the advantage of
promoting nurses within the ranks and reserving the more
menial tasks to the less experienced and younger nurses,
thereby offering a manageable workload and flexible
working ... further developing themselves and growing
within the ranks of their career. They also acknowledge
experience and time dedicated to nursing, which provides
much-needed recognition...
... determine the
potential sensitivity of studied H5N1 viruses to these anti-
virals, amino acid sequences of the M2 and NA proteins
were analyzed.
Variants of influenza A viruses resistant to amantadine
possessed ... con-
tained Glu
92
in the NS1 and contained "avian-like" PDZ-
domain ligand ESEV [19]. It was shown that the most
recent H5N1 strains isolated in Southe...
... have indicated that nucleotide
point mutations increased the yields of SaV VLPs in insect
cells, offering an alternative explanation for the increased
expression levels of rVP1 and yield of VLPs.
Results
Wt, ... substitution
in the VP1, whereas the open triangle in the VP2 gene did not
change amino acid sequence. An RNA probe (anti-VP1) was
used to monitor the...