Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
... Access
Research
Development of a real-time RT-PCR and Reverse Line probe
Hybridisation assay for the routine detection and genotyping of
Noroviruses in Ireland
John F Menton*, Karen Kearney and John G Morgan
Address: ... hospitals.
Results: A real-time RT-PCR assay and a Reverse Line Blot Hybridisation assay were developed
based on...
... found that all positives belonged to the GII/4 variant of NoV.
Conclusion: The combination of the Real-time assay and the reverse line blot hybridisation assay
provided a fast and accurate method ... Central
Page 1 of 8
(page number not for citation purposes)
Virology Journal
Open Access
Research
Development of a real-time RT-PCR and Reverse Li...
... 15
consists in a minimization of the path length, according to the Fermat
Principle. Finally, an adaptive IMR has been developed based on the
localization of reflection points.
Results obtained in a ... results of literature in a straight arch-
shaped tunnel. Then, comparisons with measurements at 5.8 GHz are
performed in a curved rectangular tunnel. Finally,...
... approval and review
processes, support for students and staff training and welfare. It has been piloted in Ghana and the
feedback was incorporated into the handbook. The handbook is currently available ... penalties for plagiarism and collu-
sion.
4. Quality assurance of approval and review processes
Aim
To maintain the academic quality of courses and ensure
th...
... Research was conducted in compliance
with the Animal Welfare Act and other federal statutes and
regulations relating to animals and experiments involving
animals and adhered to principles stated ... and Accreditation of Laboratory Animal Care
International.
Mouse adaptation
The general approach to adapt MARV to mice was based
on virus passage in scid (BALB/c background) mi...
... positive
macaques, the RV-2 assay result was low and outside the
linear range of the assay.
Discussion
We have developed a TaqMan probe- based QPCR assay to
quantitate the viral load of macaque rhadinoviruses
belonging ... from the antisense strand.
OSM-Mn CCTCGGGCTCAGGAACAAC GTC TACTGCATGGCCCAGCTGCTGGACAA CTCAGACATGA CTGAGCCCACGAAGGCC
OSM-AGM A
OSM-Human A C.G...
... design,
data collection and interpretation. PG provided intellec-
tual and theoretical input for the paper and interpretation
of the findings. All authors were involved in revising the
manuscript and ... working abroad
etc [15]. Each line on the chart would have an event
number and a start and end date. Additional information
was requested for nursing jobs, whi...
... each technique on a four-point scale (not at all satis-
Importance of ways of learning about health planning and managementFigure 1
Importance of ways of learning about health planning and management.
0.0 ... purpose of the inter-
views was to gain insights into the application and impact
of the training, enrich the findings from the questionnaire
and clarify u...
... contributions
TW, SC and IB jointly formulated the study design,
obtained and analysed the data, interpreted the findings
and wrote the article. All authors had access to all data in
the study and had final responsibility ... Mercer and Pascal Zurn, World Health Organization; and Anita
Davies and Danielle Grondin (formerly IOM), International Organization
for Migr...
... low-
income settings. A number of challenges remain, includ-
ing the ability to maintain a large volunteer workforce and
to build partnerships and collaborations with other
organizations.
Competing ... collaborate with educational and
other institutions in resource-poor settings, and hope that
through their use of Peoples-uni courses and/ or joint
accreditation of the...