Báo cáo sinh học: " Structural comparisons of the nucleoprotein from three negative strand RNA virus families" pdf
Báo cáo sinh học: " Structural comparisons of the nucleoprotein from three negative strand RNA virus families" pdf
... structure and function of the N proteins among negative strand RNA viruses.Implication of the hypothesis The structural alignments of the N proteins from three negative strand RNA virus families have ... AbstractStructures of the nucleoprotein of three negative strand RNA virus families, borna disease virus, rhabdovirus and influenza A virus, are now available. Structural comparisons showed that the topology of ... nucleoprotein of negative strand RNA viruses.Background Negative strand RNA viruses are different from all otherviruses because their RNA genomes are always enwrappedby a virally coded nucleoprotein...
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Báo cáo Y học: Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003 potx
... either one of the three inner-coreheptose residues of H. influenzae LPS [14,17,19]. The lic1locus of H. influenzae controls both the expression andphase variation of the PCho epitope [36]. The ... the steps in the biosynthesis of the oligosaccharide portion of the LPS molecules.Molecular structural studies o f LPS from mutant a ndwild-type strains of H. influenzae by us and others [10–19]have ... found in the inner-core of the LPS in one growth. Structural details of this finding are under investigation.ACKNOWLEDGEMENTS The authors would like to thank sinc erely the members of the FinnishOtitis...
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Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf
... Thusinferred from the relative rates of fragment formation, the cleavages of the sites were in the order: Tyr146/Asn147 !Phe114 ! other sites. The weakness of bands of fragments Iand IV and the appearance ... appearance of fragment II in the degradation of the Tyr146!His/Asn147!Ser mutant (panel d)indicated a significant decrease in the rate of cleavage of the autolysis loop. At the same time the amount of ... was the same as seen from their inactivation rates in the presence of equimolaramounts of trypsin (Table 2). The cleavage of the interdomain and autolysis loopsTo follow the autolysis of D-chymotrypsin...
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Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx
... responsible, from a thermodynamic point of view, for the binding of the monomeric species of CAC?We have previously discussed the variation in the freeenergy of binding as a function of the changes ... relationshipbetween S2and the location of structural elements(Fig. 4). Furthermore, the Rexterms are distributedthroughout the 3D structure of the protein, and most of them are large (Fig. 4); the exception ... the product of the corre-lation function for overall (global) and internal (local)motions (i.e. the internal motions of the bond vectorsare independent of the overall rotational movement of the...
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Báo cáo khóa học: Structural properties of the protein SV-IV potx
... identified by the massincrease of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species. From the data ... Lys80,showed partial acetylation at one of the three residues,because the signal of the triacetylated species was lessintense than that of the diacetylated species. The MS/MSFig. 2. Secondary-structure ... estimated that the a-helixcontent is 24% in the monomeric form and 45% in the trimeric form, and b-structure is absent from both forms. The CD spectra of the 1–70 and 70–90 regions of the proteinare...
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Báo cáo khoa học: Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognition pptx
... diminishedFig. 5. Model of the acceptor end of tRNASerbound in the activesite of mMbSerRS. The view is focused on the acceptor part of tRNA in the active site of mMbSerRS. The tRNA is shown as anorange ... Switzerland The fidelity of protein synthesis depends on the correctattachment of amino acids to the 3¢-ends of their cog-nate tRNA species by aminoacyl-tRNA synthetases(aaRS), a family of enzymes ... 2008 The Authors Journal compilation ª 2008 FEBS 2843ogy modeled based on the structure of the E. coliThrRS loop. We observed that the positioning of the cognate tRNA in the active site of mMbSerRS...
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Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot
... study the secondary structure of the catalytic domain of PKCfand the effect of its substrate, MgATP, and also tostudy the effect of thermal unfolding in the presenceand the absence of MgATP. ... structure of 282 residues of a total of 344have been solved [19]. The catalytic domain of PKChpossesses a high degree of homology ( 80%) with the catalytic domain of other isoforms of PKC belongingto ... early separation of the branch of the atypical PKCs from other proteinkinases in the evolutionary tree [43].It is, however, striking that, besides the lack of sequence homology, there is remarkable...
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Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt
... functional studies of Nog o-A.On the other hand, the recent identification of Nogo-40as a potent NgR antagonist suggests a promising startingpoint for the design of potential therapeutic agents ... Zn2+.Furthermore, the d etermination of the Nogo-40 solutionstructure offers a starting point for further understanding the interaction between NgR and Nogo-40, and forfuture designs of molecules ... stabilized by the introduction of the helix-stabilizing solvent trifluoroethanol.We report here the structure of Nogo-40, a Nogo-66Fig. 1. Schematic representation of the doma in organization o f the...
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Báo cáo khoa học: Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 potx
... of note that the structure of the LPS O-antigen of F. columnare differs structurally from the LPS O-antigen of the fish pathogen Flavobacterium psychrophilium [21],which is a linear polymer of ... beingcomposed of aL-FucN component originating from the O-PS residue A and the D-FucN from the reduced residue B. The isolation of optically pure 2-amino-2,6-dideoxy-D-glucose (D-QuiN), the major ... (1.68 g),which was used in all further studies. The addition of acetone (6 vol.) to the supernatant from the above ultracentrifugate remaining after the collection of LPS afforded a precipitate (94...
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Báo cáo khoa học: Structural characterization of the large soluble oligomers of the GTPase effector domain of dynamin potx
... for formation of short helices, transiently,and the chain as such is highly dynamic.Structure of the core of the oligomersBecause of the large size of the core of the oligomer, the NMR spectra ... signals from the interior of the core and thus do not give any informa-tion on its structural details. Nevertheless, we didderive useful insights into the structural aspects of the core of the ... calculated the electrostatic potential of the surfaces of the twolong helical segments predicted from the above algo-rithms; these are shown in Fig. 8. The two oppositefaces of the helices...
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