... immu-noglobulin hypervariable domains, intra-chain disul-phide bonds can, to varying degrees among differentantibodies, influence conformation of the antigenbinding site [31]. Thus, failure to form intra-chaindisulphide ... adapted to promote formation of intra-chaindisulphide bonds in vitro, using protein extracted frombacterial inclusion bodies. After isolation of inclusionbodies by sonication of bacteria, proteins ... infection in vitro, inhibits cell -to- cell spread of virus and can be used to prevent infection in a guinea pig model of GH.ResultsConstruction and expression of single chain antibodies against...