... Transwell insert (24-well insert; poresize, 8 lm; Corning, Inc., Corning, NY, USA) assay toevaluate PC9 cell migration and invasion in vitro. In both the migration assay and the invasion assay, ... go awry in human cancers.Experimental proceduresCells and cultures The human lung cancer cell line A5 49 was obtained from the American Type Culture Collection (Manassas, VA,USA) and maintained ... USA), and antibody againstphospho-Akt was from Santa Cruz Biotechnology, Inc.(Santa Cruz, CA, USA).Western blot analysis To examine the in uence of gefitinib on phosphorylation of proteins,...