báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt

... uncontaminated. To assure viabil-ity and sterility, a vial was recovered from the freezer and passaged 5 times and then tested for mycoplasmal, bacte-rial and fungal contaminants. Six cultures are currentlyavailable ... citation purposes)Journal of Translational MedicineOpen Access Methodology Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and ... testing.Results: Fibroblasts from the non-healing edge had almost no migration capacity, wound base fibroblasts were intermediate, and fibroblasts derived from the healing edge had a capacity to migrate similar...

báo cáo hóa học: " Primary Sjögren''''s Syndrome: health experiences and predictors of health quality among patients in the United States" potx

... hospitalizations within the past 5 years, dental visits and costs of dental care as well as the overall impact ofSjogren's on activity, family and social life. The ASSESSquestionnaire also ... demonstrated that with the exception of oraldamage, end organ damage was uncommon in primary SS, yet the degree of functional disability was as great in patients with primary SS as in those with SLE[3].Strombeck ... health quality and excess health costs are prevalent among PSS patients. Health experiences and functional impact of PSS is similar among US and European patients. Delayed diagnosis, sicca related...

báo cáo hóa học:" Development of targeted therapy for ovarian cancer mediated by a plasmid expressing diphtheria toxin under the control of H19 regulatory sequences" doc

... transfection of human cell lines with Luc-H19 plasmid and the reduction of luciferase activity in human ovary primary culture from patient #1 and in human ovarian carcinoma cell lines due to ... therapeutic potential and is a very promising candidate for ovarian cancer ther-apy in humans.Materials and methods Cell cultureThe human ovarian carcinoma cell lines (ES-2, SKOV-3,TOV-112D and ... indicated vectors and luciferase activity wasmeasured by luciferase assay (Figure 3A) . Next we alsotested the in-vitro killing potential of the DTA-H19 plas-mid in the same human ovarian cancer...

báo cáo hóa học:" Rapid serological detection of autoantibodies associated with Sjögren''''s syndrome" docx

... point-of-care testing because theyare complex, time consuming, and difficult to standardize[3]. As an alternative, rapid tests such as lateral flowimmunoassays, which can more easily be integrated ... Section, Laboratory of Sensory Biology, National Institute of Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA and 2Division of Rheumatology and Clinical Immunology and ... additional autoantigens revealed that certain SjS patients also showed positive immunoreactivity with Ro52, Ro60 and other extraglandular autoantigensincluding thyroid peroxidase, the aquaporin-4 waterchannel...

báo cáo hóa học:" Comparison of the effects of vitamin D products in a psoriasis plaque test and a murine psoriasis xenograft model" docx

... 4Immunohistochemical stainings of a keratome biopsy before (A, B and C) transplantation and after (D, E and F) transplantation and treatment in the psoriasis xenograft SCID mouse. Before transplantation ... not significant.Immunohistochemical stainings of a keratome biopsy before (A, B and C) transplantation and after (D, E and F) transplantation and treatment in the psoriasis xenograft SCID mouseFigure ... H and K) and Daivobet® (C, F, I and L) in the psoriasis plaque assay. A- C: Masson-Trichrome (MT) staining showing epidermal hyperplasia. D-F: Proliferation of keratinocytes in stratum basale...

Báo cáo hóa học: " Boosting high-intensity focused ultrasoundinduced anti-tumor immunity using a sparse-scan strategy that can more effectively promote dendritic cell maturation" ppt

... radiotherapycan express ‘danger’ and ‘eat me’ signals s uch as heat-shock proteins (HSPs) on the cell surface or releaseintracellular HSP molecules to stimulate DCs to mature and elicit a strong anti-tumor ... studies was not strong enough to achieve a therapeutic gain. As mentioned above, localtumor recurrence and distant metastasis are often thecause of failure for HIFU therapy[ 10,12], indicating ... (Gibco, USA) at37°C and 5% CO2.Experimental animals and Tumor ModelC57BL/6 female mice, 5-8 weeks old, were purchased from Shanghai SLAC Laboratory Animal CO. LTD (Shanghai ,China). Tumor...

Báo cáo hóa học: "The density of macrophages in the invasive front is inversely correlated to liver metastasis in colon cancer" docx

... Saito K, Shiiba K, Sasaki I, Matsuno S, Yagita H,Miyazawa M, Nagura H: Close association between Fas ligand (FasL;CD95L)- positive tumor-associated macrophages and apoptotic cancercells along ... CD4(+) T cells regulate pulmonary metastasis of mammarycarcinomas by enhancing protumor properties of macrophages. Cancer Cell 2009, 16(2):91-102.48. Mantovani A, Sica A, Allavena P, Garlanda C, ... CD68TFHotspot and the clinicopathologic parameters, thepotential of hepatic metastasis, and the 5-year survival rate were analyzed.Results: TAMs were associated with the incidence of hepatic metastasis and...

Báo cáo hóa học: " Prognostic impact of ZAP-70 expression in chronic lymphocytic leukemia: mean fluorescence intensity T/B ratio versus percentage of positive cells" pot

... the Alexa-488-conjugated anti-ZAP-70 mAb with an isotype-matchedAlexa-488-conjugated control mAb (Caltag). All sampleswere acquired on a FACSCanto flow cytometer and ana-lyzed with DiVa software ... Burlingame,CA) according to the manufacturer’s instructions, and finally stained with the Alexa-488- conjugated anti-ZAP-70 mAb (clone 1E7.2, Caltag). A second tube was p re-pared exactly as above, ... ratio was calculated directly from MFI values as separately read from T cell and CLL cell gates defined in panel A. Rossi et al. Journal of Translational Medicine 2010, 8:23http://www.translational-medicine.com/content/8/1/23Page...

Báo cáo hóa học: " Development of targeted therapy for bladder cancer mediated by a double promoter plasmid expressing diphtheria toxin under the control of H19 and IGF2-P4 regulatory sequences" potx

... the RNA STAT-60TM Total RNA/mRNA isolationreagent, according to the manufacture’s instructions. TheRNA was treated by RNAse-free DNAse I t o eliminateany contaminating DNA. Total cDNA was synthesized from ... determined and the mean of the total areas was calculated for each group. The Mean and SD of bladder tumor area (A) and weight (B) are shown.Amit and Hochberg Journal of Translational Medicine ... Superficial, invasive and metastatic.At presentation, 75% of the tumors are superficial, 20%are invasive and up to 5% have de no vo metastasis. Thewall of the bladder is lined with cells called transitionalcells....

báo cáo hóa học: " Retraction: Psychometric characteristics of the ankylosing spondylitis quality of life questionnaire, short form 36 health survey, and functional assessment of chronic illness therapy-fatigue subscale" pptx

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