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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx

Báo cáo y học:

Báo cáo y học: "In vitro bactericidal activities of Japanese rice-fluid against Helicobacter pylori strains"

... in human infections, including H. pylori strains. 2. Materials and Methods Bacterial strains In this study, two standard strains, that is ATCC4350 (type strain) and ATCC43526, and 14 clinical ... pylori infection. Some investigators have documented that various medicinal plant extracts confirm antibacterial activities [20-24]. We investigated the in vitro antibacterial activity of hitherto ... against H. pylori infection. The mechanisms in detail of bactericidal activity against the three species, especially H. pylori strains remain to be elucidated. Conflicts of interest The authors...
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Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf

Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf

... repeatsare relatively better than for ATT, CTG and GT.State of the template during slippage extensionSeveral repeated templates with 5¢-32P-end labelingwere used to investigate template extension. ... stability is attrib-uted to the T T mismatch which stacked more effi-ciently on the CTG strand than the AÆA mispair onthe complementary CAG strand, resulting in expandedCTG fragments that are ... system, theexpanded primary bands were almost all seen in the three-nucleotide unit, which indicated that the in vitro DNA strand slippage synthesis of (ATT)3⁄(AAT)5tract was mainly a triplet...
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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

... other factors tobind to the CT element and, thus, modulate CT ele-ment activity. Here we report that in vitro phosphory-lation does not modify Comp-CT binding capability.Instead, it promotes ... of the CT element from the human c-myc promoter,opposite to hnRNP K that binds to the pyrimidine-rich strand. These interactions determine the formation of an open complex at the CT element composed ... structure remodelling activity and, conse-quently, might affect the translational control. In the other reported model for CNBP function,CNBP was found binding to the purine-rich strand of the...
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Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx

Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx

... c-secretase was diluted to 0.25% CHAPSO and 2, 1, and 0.5 lg of extract tested for activity. Note that faint activity was seen with 1 lg extract.Characterization of in vitro c-secretase activity ... transmembrane substrates.This is supported by our work and by other studiesshowing its activity is highly sensitive to factors thatmodulate membrane structure and stability, inclu-ding detergent type ... stabilized by dimethylsulfoxide in aconcentration-dependent fashion.Our results indicate that in vitro c-secretase cleavage of APP substrates is inhibited by zinc and that zincincreases the...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc

Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc

... AnI19R5¢-AATTCATGAGGAGGTTTCTCTGTAACA-3¢;AnI19H5¢-AGCTTGTTACAG-AGAAACCTCCTCATG-3¢; AnI17R5¢-AATTCACGAGGAGGTTTCTCTGTACTA-3¢;AnI17H 5¢-AGCTTAGTACAGAGAAACCTCCTCGTG-3¢;AnI15R5¢-AATTCACAGGAGGTTTCTCT-GTCTA-3¢;AnI15H5¢-AGCTTAGACAGAGAAACCTCCTGTG-3¢. ... Furthermore, the fact that the proteinwas saturated with the same amount of either substrate(Fig. 4) argues against the trivial explanation that only afraction of protein was competent to bind ... sub-strate fails to inhibit RNA splicing activity. The data suggesttherefore that both nucleic acids do not bind the same singlebinding site, rather that I-AniI appears to contain twobinding...
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Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot

Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot

... activity to cleave a nativesubstrate prestreptokinase and a peptide substrate, indicat-ing that the self-cleavage inactivates the enzyme. Moreimportantly, we found that the self-cleavage of ... also happening in vivo in all the growthphases, and that the bacterial cells maintain the activeSPase I at the highest level in the exponential growth phase.These results suggest that the self-cleavage ... (5¢-GCAATGTTCGCGTACATATGCATTCCATGGATCCGACC-3¢ and 5¢-TGGTGGTGCTCGAGAAATGTTCCGATACGGGTGATTGGCCAGAAGCG-3¢), which were designed to contain NdeIandXhoIrestriction sites at the 5¢ ends, respectively. The primers...
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Báo cáo Y học: In vitro gene therapy of mucopolysaccharidosis type I by lentiviral vectors pdf

Báo cáo Y học: In vitro gene therapy of mucopolysaccharidosis type I by lentiviral vectors pdf

... totreatment of mucopolysaccharidosis type I because theyimply that the introduction of a therapeutic IDUA gene by lentiviral vector into a small portion of target cells may result in the release of the ... prepared using aconditional packaging system [23]. The proviral form of thevector is shown in Fig. 1. The packaging system containsthe self-inactivating transducing constructs (sin) obtainedafter ... catabolism after treatmentwith vector.Metabolic labeling of recombinant IDUA enzyme in transduced MPS I fibroblastsThe maturation of the recombinant IDUA enzyme wasstudied through metabolic labeling...
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Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx

Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx

... somewhat theinterpretation of the folding result. The experimentalselection and characterization of trPRAI therefore con-stitutes support for the identity of the putative(ba)1)5b6folding intermediate ... probably attributable to the removal of a tryptophanresidue (Trp391) in the truncation.The stability of trPRAI-His to thermal denaturationwas investigated by monitoring ellipticity at 219 nm(Fig. ... survive multiple rounds of in vitro selection suggests that this result is unlikely tobe common to the other loops. Experiments to test themutability of the remaining active-site loops in PRAIhave...
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Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot

Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot

... homologous quinone in this electrontransfer chain and, out of all tested quinones, it is the onewhich most efficiently incorporated into it (judging from itsstrong stimulation of respiratory rates; ... of the increase in the overall quinone reducing activity of the membranes and not a direct effect on PTOX activity. This is corrobor-ated by the fact that the ratio of O2consumption before and Fig. ... inhibitors are also of interest as they would allowinactivation of PTOX at a certain time during plantdevelopment without pleiotropic effects that can beassociated with genetic mutants. When...
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Báo cáo khoa học: Betulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expression pot

Báo cáo khoa học: Betulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expression pot

... replication.ResultsBetA-induced selective cytotoxicity in HBV-infected hepatocytesWe first examined the cytotoxicity of BetA in wild-type (WT) and HBV-infected hepatocytes. Differentdosages of BetA were used to ... decreased in total lysates and cytosolic frac-tions but increased in mitochondrial fractions afterBetA treatment, and siCREB mimicked the effect of BetA, indicating that BetA treatment not only ... BetA-induced HBV inhibition, suggesting thatthe BetA-mediated redox effect could be both benefi-cial and detrimental to cells; the most important thingis to maintain a balance that achieves the...
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