... polymerase a- primase complex during meiosis in Coprinus cinereusSatoshi Namekawa, Fumika Hamada, Tomoyuki Sawado†, Satomi Ishii, Takayuki Nara‡, Takashi Ishizaki,Takashi Ohuchi, Takao Arai and ... mMb-mercaptoethanol, 50% glycerol, 0.01%Nonidet P-40, and 20% sucrose.DNA primase assayTheDNAprimaseassayusingaDE81filterwasthesameas the DNA polymerase assay except that the RNA primingactivity ... I.DiscussionBiochemical features of pol a- primase complexduring meiotic stages In this examination of the DNA polymerase a- primasecomplex, we determined the molecular mass of the pol a catalytic subunit and the...