... salad
bowl a layer of slightly broken chips, then half of the chicken pieces, and pour half of the
Rotel mixture over the chicken. Repeat with another layer of each, then top it off with
more crumbled ...
Nice to serve with fresh fruit and coffee cake;
everyone gets involved in the process and a
great conversation piece
Quickie Stoganoff
brown a pound or so of ground bee...
... and
substrate-binding amino acid affected the binding of
dThd and deoxycytidine (dCyd).
Results
We performed site- directed mutagenesis of four active
site residues of Dm-dNK. Residue E52 was mutated to
D, ... kinetic properties of
the active site mutants are summarized in Table 1. All
mutants were characterized with dThd and dCyd,
Fig. 2. Binding of the substrate dThd at the a...
... description of the exact role, elec-
trostatic properties, and hydrogen bonding potentials
of active site residues provides an optimized model of
the ligand-binding potential of the active site, enabling
differentiation ... to visualize acyl-ACPs. The lag
A
B
Fig. 5. The active sites of the K328R mutant
protein. (A) Active site of the
C monomer;
(B) active site of the
D m...
... (22.7) 8.8 (24.5)
Number of protein atoms 2083 2081 2080 2079
Number of carbohydrate atoms 57 57 71 57
Number of sulfate ions 1 1 3 1
Number of glycerol molecules – – 5 –
Number of water molecules ... influences the active site properties. The cause of
these differences is not known.
K
m
and
k
cat
measurements of hevamine and mutants
Comparison of the steady-state kinetic par...
... and
temporal expression of specific subsets of genes. The
sequential silencing of genes involved in maintenance
of pluripotent and multipotent states and the activa-
tion of those involved in differentiation ... organisms are composed of a variety of
cell types, all derived from a common precursor and
identified by different patterns of gene expression. It is
the transcriptional...
... residue and enable
the active site of DspB to be flexible. Differences
R27
E332
W330
W237
W216
Y278
E184
A
B
D183
GlcNAc
Fig. 5. Active site of DspB. (A) Active -site
residues of DspB and their potential
interactions ... G. A. Manuel et al. Active site of dispersin B
FEBS Journal 274 (2007) 5987–5999 ª 2007 The Authors Journal compilation ª 2007 FEBS 5999
Role of active -site...
... Under these conditions, 1 unit of the enzyme
was defined as the amount of enzyme catalyzing the produc-
tion of 1 lmol of NADH per min. An extinction coefficient
of 6.22 per mm for NADH was utilized ... [4,5,31–33]. In the structure of human
m-NAD-ME, besides the active site, there are two
regulatory sites (Fig. 1A). One of them, located at the
tetramer interface and called the e...
... of the Asn624 site
The MALDI-TOF analysis of the different peptide fractions
collected after RP-HPLC fractionation of tryptic hydroly-
sates allowed us to detect the potential glycosylation site
Asn624. ... Asn624 site.
Structural analysis of N-glycopeptides
Glycopeptide-containing fractions were analysed by
MALDI-TOF before and after N-glycanase treatments.
Fig. 1. Fractionatio...
... trees swaying. While car camping,
there is usually a communal restroom that almost always smells of
disinfectants or deodorants.Car camping and backpacking are two forms
of camping that differ from ... To get to the campsite while car camping, one
follows a road map to the park, drives to the ranger station, picks up a
pass, and pulls in to the campsite. Depending on the size of...
... types of spacer
fractures and dislocations and offers some suggestions about reconstructive techniques for
management of extensive loss of femoral and/or acetabular bone at the site of hip ... implantation technique also offers the ad-
vantage of a prevention of a spacer migration into the
pelvis (Figure 4) beside a normal articulation and
prevention of any spacer dislocat...