... for the extraction of bioflocculants from activated sludge, followed by steaming extraction. SDS and washing extraction methods were ineffective because of low flocculating activity, and low ... Journal of Water and Environment Technology, Vol.3, No.1, 2005 - 145 - Comparison of Methods for the Extraction of Bioflocculants from Activated Sludge Junichi TSUGE1, Masuo NAKANO2 and ... by extraction. Several investigators have isolated bioflocculants from pure cultures of microorganisms (Takagi and Kadowaki, 1985a, b; Kurane et al., 1986; Bar-or and Shilo, 1987; Kurane and...