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Single cell protein introduction

Single cell protein introduction

Single cell protein introduction

... Single cell protein introduction Single cell protein conceptSingle cell protein concept Single cell protein is a terms for a type of cell nutrient and it produced ... of single cell protein synthesis as: bacteria, yeast, filamentous fungi, algaeYeast BacteriaAlgae Filamentous FungiAdvantages and disadvantages• Advantages of single cell protein: Protein ... of single cell protein: Protein in high cell Good quality protein Safety toxins• Disadvantages of single cell protein: • Slow digestion by the cell structure• High nucleic acids produce...
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Báo cáo khoa học: Bacterial IscU is a well folded and functional single domain protein pdf

Báo cáo khoa học: Bacterial IscU is a well folded and functional single domain protein pdf

... ions, it is probable that theformation of Fe–S clusters is mediated by protein protein interactions. NifS and NifU, the specific proteins involvedin the building of Fe–S clusters were originally ... two additional amino acids (GlyAla) atthe protein N-terminus. The constructs were expressed inE. coli strain BL21(DE3). For protein expression, the cellswere inoculated in Luria–Bertani medium ... equipped with a cell holder thermostatted byaPTC-348Peltiersystem.FarUVmeasurementswereperformed in 10 mMbuffer at pH 7.5 using protein concentrations of 7–35 lMfrom two independent protein preparations....
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Báo cáo Y học: Modelling of simple and complex calcium oscillations From single-cell responses to intercellular signalling pdf

Báo cáo Y học: Modelling of simple and complex calcium oscillations From single-cell responses to intercellular signalling pdf

... However, cellularCa2+transients also have a spatial dimension. In thecytoplasm of single cells, C a2+gradients can be observedwhen Ca2+release from the ER is excited at particularsubcellular ... the cells oscillating faster in isolationpeak before the slower cells. Thus the intercellular couplingleads to a (near) 1 : 1 entrainment, or synchrony, of theoscillations in adjacent cells. ... [197]. Intercellular entrainment of Ca2+oscillationshas also been observed i n other cell types, such aspancreatic acinar cells [198], articular chondrocytes[199,200], kidney cells [201],...
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Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx

Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx

... (a1vector)inCHO-K1cells. Cells were allowed to grow for 10 days before protein expression was analysed. Cell fusion Cell fusion was performed in two individual steps (Fig. 1) bymixing equal number of cells ... each protein subunit. The cell- fusion technologydescribed allows a predefined expression level of each sub-unit. Using SIgA as a model protein we demonstrate that themajority of the fused cells ... of individually transfected cells,results in a fused cell- line that secretes a complete multi-subunit protein. Functional expression of recombinantmultisubunit proteins may require a defined...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt

Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt

... expression, cells may dieduring antibiotic selection because of toxic effects ofthe expressed protein [1]. Also, for a close match ofbackgrounds, it is better to compare two states of a single cell ... for transport proteins,pools of transfected human embryonic kidney 293 cells showed on ⁄ offmRNA ratios in the order of 100 : 1. Cell pools were also analyzed forregulation of protein function. ... EBNA-1 protein arecontinuously propagated in 1% of initially transfect-ed cells [4]. EBV plasmid replication has been demon-strated for a large variety of human cell lines; primateand canine cell...
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Tài liệu Báo cáo khoa học: Evolutionary divergence of valosin-containing protein ⁄cell division cycle protein 48 binding interactions among endoplasmic reticulum-associated degradation proteins ppt

Tài liệu Báo cáo khoa học: Evolutionary divergence of valosin-containing protein ⁄cell division cycle protein 48 binding interactions among endoplasmic reticulum-associated degradation proteins ppt

... thisfundamental cellular process. For example, valosin-containing protein (VCP) and its yeast homologue cell division cycle protein 48 (Cdc48p),which help to direct polyubiquitinated proteins for ... recombinant proteins and proteins fromHeLa cell extract.Fig. S3. Parts (L)–(S) of Fig. 3 to demonstrate Ube4bintrunuclear puncta more clearly.Table S1. Details of the VBM or putative VBMs inERAD proteins ... FEBSEvolutionary divergence of valosin-containing proteincell division cycle protein 48 binding interactions amongendoplasmic reticulum-associated degradation proteinsGiacomo Morreale, Laura Conforti,...
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Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf

Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf

... distribution of G2⁄ M cells: thenumber of metaphase cells with a single DNA mass atthe bud neck and the number of anaphase cells wereincreased slightly in the mutant (metaphase cells: 30.2%in the ... [11].Keywordsatypical protein kinase; casein kinase 2substrate; cell- cycle phase-dependentdegradation; protein protein interactions;Rio1 protein kinase21CorrespondenceM. AngermayrE-mail: M.Angermayr@lrz.uni-muenchen.dePresent ... (2002) Joining the cell survival squad: an emerging role for protein kinaseCK2. Trends Cell Biol 12, 226–230.3 Meggio F & Pinna LA (2003) One-thousand-and-onesubstrates of protein kinase CK2?...
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Tài liệu Báo cáo khoa học: Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r ppt

Tài liệu Báo cáo khoa học: Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r ppt

... andincubated with the cells for 1 h at room temperature. Cellswere washed, mounted and analyzed as described above.To semipermeabilize cells for intracellular staining, cellswere incubated ... human genome. Cell 117, 699–711.14 Van Vactor D (1998) Protein tyrosine phosphatases inthe developing nervous system. Curr Opin Cell Biol 10,174–181.15 Stoker A & Dutta R (1998) Protein tyrosinephosphatases ... orthologs of receptor protein tyrosine phosphatase-r are alsoimplicated in the recognition of muscle target cells. A potential extracellu-lar ligand for vertebrate receptor protein tyrosine phosphatase-r...
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Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx

Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx

... intracellular localizations ofthe human ER and PDIP46/SKAR proteinsWe examined the intracellular localization of thehuman ER and PDIP46 ⁄ SKAR proteins fused withenhanced green fluorescent protein ... human ER protein. The screen yielded polymerase d interacting protein 46 or S6K1Aly ⁄ REF-like target (PDIP46 ⁄ SKAR), a protein possessing one RNArecognition motif (RRM) and being a protein ... that excluded the nucleoli,in both HeLa and NIH ⁄ 3T3 cells (Fig. 4A–C). In thesame cells the mitogen-activated protein kinase orextracellular signal-regulated kinase 1 (MEK1) pro-tein fused...
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Tài liệu Báo cáo khoa học: Cell-free translation systems for protein engineering docx

Tài liệu Báo cáo khoa học: Cell-free translation systems for protein engineering docx

... transformation in cells, and overexpres-sion of the desired protein in cells. Thus, there arelimitations associated with using in vivo technology for protein production. Cell- free translation ... engineering.AbbreviationsEGFP, enhanced green fluorescent protein; GFP, green fluorescent protein; PDI, protein disulfide isomerase; PURE, protein synthesis usingrecombinant elements; scFv, single- chain variable fragment ... relatingto cell- free translation systems for enhancing the syn-thesis of biologically active proteins, the creation of cell- like compartments and the synthesis of artificialproteins.Overview Cell- free...
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