molecular genetics of mammalian cells
molecular genetics of mammalian cells
... properties of growing $49 cells. (1) The ability of cAMP to arrest wild-type but not cAMP-resistant mutant cells in the Gt phase of the cell cycle. (2) The extreme sensitivity of cells to white ... against losing cells that have been defrosted is to freeze them in 10% glycerol. Although survival of cells frozen in glycerol is not as good as that of cells frozen in DMSO, cells frozen and ... hr. Mutagenesis of CHO Cells For most selections, it is necessary to mutagenize cells to get a reason- able frequency of mutants. Because of its relative stability and ease of handling, we...
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Báo cáo khoa học: Recent insights into cerebral cavernous malformations: the molecular genetics of CCM pot
... acomplete loss, within affected cells, of the two alleles of a given CCM gene. Loss of one of the alleles (firsthit) would be the result of a germline mutation andloss of the second allele (second ... sequencing of lesion DNA, emphasizingthe lack of sensitivity of direct sequencing of lesionDNA. These data established the existence of biallelicsomatic and germline mutations, whatever the nature of ... to test because of theheterogeneous cellular nature of CCM lesions and thevery limited number of endothelial cells lining the cap-illary cavities. Indeed, direct sequencing of the DNAextracted...
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Báo cáo Y học: Growth inhibition of mammalian cells by eosinophil cationic protein pptx
... A431 cells, MDA-MB-453 cells, HL-60 cells and K562 cells were esti-matedtobe% 1±5 lM. ECP signi®cantly suppressed thesize of colonies of A431 cells, and decreased K562 cells inG1/G0phase. ... inhibition of K562 cells in Fig. 2 wasshown after 7 days of treatment with ECP, which wasFig. 2. Suppression of cell growth in the presence of ECP. Left, the percentages of viable cells under various ... dependent oncell type. The growth of four of the seven human cell linesFig. 3. Growth-inhibitory eect of ECP on K562 cells. (A) Cells wereseeded at 25 000 cells per 35-mm dish. Simultaneously,...
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Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx
... stimulation of ERK1 ⁄ 2 andPak1 pathways, gefitinib might lead to inhibition of invasiveness of human cancer cells through the inhibi-tion of ERK1 ⁄ 2 and Pak1. The use of gefitinib in cells with ... tumor development: molecular basis of responsiveness of cancer cells to EGFR-targetingtyrosine kinase inhibitorsKenji Takeuchi and Fumiaki ItoDepartment of Biochemistry, Faculty of Pharmaceutical ... phos-phorylation state of their proteins, thereby changingthe amount and localization of Bcl-2 family members.However, each type of cancer has its own way of disrupting the balance of the networks of signalingcascades...
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Tài liệu Báo cáo khoa học: Mammalian mitotic centromere-associated kinesin (MCAK) A new molecular target of sulfoquinovosylacylglycerols novel antitumor and immunosuppressive agents pptx
... [1,2]. However, the molecular targets of SQAGsare ambiguous. The aim of the present study was toidentify molecular targets that could be of significance.Earlier, effects of SQAGs were found ... inthe chemical synthesis of a number of forms. We havefound a-anomers that possess potent antitumor activ-ity but do not have many of the serious side-effects of Keywords mammalian DNA polymerases; ... originally as inhibitors of mammalian DNA polymerases. However, SQAGs can arrest cultured mammalian cells not only at S phase but also at M phase, suggesting theyhave several molecular targets. A...
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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells pdf
... by FACS of living cells expressing lowlevels of antigen.DiscussionThe potential of scFvs for demonstrating the presence of targeted protein antigens in the cytoplasm of mam-malian cells is ... significant difference of number of highly fluorescent cells in these cell samples as evidenced by the amount of cellcounts in the 102)104range of fluorescence intensity. (B) COS cells were cotransfected ... theamplification of the 3¢ end of the b-gal gene. The presence of similar amounts of genomic DNA in the reactions wascontrolled by concomitant addition of primers that amplifya 200 bp region of the RNA...
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Báo cáo khoa học: Functional characterization of ecdysone receptor gene switches in mammalian cells docx
... 1.647 nm (confidenceinterval of )0.5963 to 3.891, R2 of 0.9292, degree of freedom of 10) and 1.447 nm (confidence interval of 0.9588–1.935, R2 of 0.9788, degree of freedom of 28),respectively. These ... DiscussionThe most significant contributions of this study arebetter understanding of the molecular mechanism of EcR gene switches in mammalian cells and develop-ment of an efficient EcR gene switch that ... characterization of ecdysone receptor geneswitches in mammalian cells Siva K. Panguluri1, Prasanna Kumar2and Subba R. Palli11 Department of Entomology, College of Agriculture, University of Kentucky,...
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Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
... Linearity of DAPI-stained DNA content of a nuc-leus was preserved throughout such treatments; i.e. theDNA content of G2, M cells are almost double that of G1 cells and the DNA content of S cells ... re-replication of DNA in a single cell cycle. Inactivation of CDK1 leadsto re-replication of DNA in eukaryotic cells includinghuman cells [7]. Targets of the kinase in the regulation of DNA replication ... ⁄ 328) of cells, and in those thatdo express the CDK2, strong signals were observed in1.6% (2 ⁄ 129) of cells. For P-32 antibodies, strong sig-nals were detected in 42% (100⁄ 238) of cells...
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Báo cáo khoa học: Identification of sodium salicylate as an hsp inducer using a simple screening system for stress response modulators in mammalian cells pptx
... transcription of hsp genes and the accumu-lation of Hsps in various mammalian cells and a concomi-tant increase of thermoresistance of cells. Thus, SA maybe used for the protection of cells against ... such as Hsp105a and Hsp70 in various mammalian cells. Enhancement of thermoresistance of cells by SAUpon exposure to a sublethal heat treatment, mammalian cells acquire transient resistance to ... and p173OR/C3H cells, and pGL105/C3H cells seemed to be more useful forscreening modulators of stress response in mammalian cells. Induction of stress response by SAEnhancement of heat shock...
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Báo cáo Y học: Molecular cloning of columbamine O-methyltransferase from cultured Coptis japonica cells pot
... (R)- and (S)-coclaurine werethe gift of Dr Nagakura of Kobe Women’s College of Phar-macy, and (S)-reticuline was a gift from Dr Facchini of theUniversity of Calgary. The other alkaloids were ... of columbamine wasapproximately 8.4. Whereas product inhibition of 6OMTand SMT has been reported [8,17], berberine, one of theend-products of isoquinoline alkaloid biosynthesis in Coptis cells, ... characterization of coclaurineN-methyltransferase from cultured cells of Coptis japonica. J. Biol.Chem. 277, 830–835.15. Frenzel, T. & Zenk, M.H. (1990) Purification and characterization of three isoforms...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam