... containing L-AMP,LUC, CoA and CoA analogues. Reaction mixtures containing L-AMP(20 lM), LUC, and CoA or the indicated CoA analogues were incu-bated for 10 min. After stopped by the addition ... converted the resi-dual CoA present in the commercial acetyl-CoA intoacetyl-CoA preincubating it with ATP, acetate, MgCl2,acetyl-CoA synthetase and inorganic pyrophosphatase(PPase). Then, we ... luciferase. Anal Biochem 95,194–198.30 Zako T, Ayabe K, Aburatani T, Kamiya N,Kitayama A, Ueda H & Nagamune T (2003)Luminescent and substrate binding activities of fireflyluciferase N-terminal...