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structural levels of the iliad

structural levels of the iliad

structural levels of the iliad

... levels to the war. These structural levels are of special interest, because they help define the consequences and outcomes of the war. The universal war of the gods, social war of the Greeks ... Trojans, and the private war of Achilles' honor are structural levels of the Trojan war. These structural levels seem to influence and shape each other in many distinct ways. The universal ... KingMenelaus. The abduction of Helen by Paris lead to the Trojan war. The promise made by Aphrodite to Paris in order to get the apple of discordresulted in the abduction of Helen and the start of the...
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx

Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx

... b ath for the required amount of time. At the end of the heattreatment the mixtures were placed in an ice/water bath,and the enzymatic activity w as stopped by lowering the pH of the r eaction ... BLG/protease mixture was then placed in a water bath thermostatted at 60 °C for the given amount of time . At the end of the heat treatment the mixture s were placed o n ice, and the enzymatic activity ... with the remainder of the BLG structure. It is evident that a ll these fragmentsinclude a generous portion of the b-barrel structure (strandsB, C and part of strand D), along with part of the...
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Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf

Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf

... from the relative rates of fragment formation, the cleavages of the sites were in the order: Tyr146/Asn147 !Phe114 ! other sites. The weakness of bands of fragments Iand IV and the appearance of ... other; and(c) the hydrolysis of the Phe114-Ser115 bond was the ratedetermining step in autolytic inactivation. Thus, it is the cleavage of the interdomain loop and not of the autolysis orother ... was the same as seenfrom their inactivation rates in the presence of equimolaramounts of trypsin (Table 2). The cleavage of the interdomain and autolysis loopsTo follow the autolysis of D-chymotrypsin...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

... were inserted between the third letter of one codon and the first letter of the following codon.Molecular modeling of the C. sepiumb-amylase The amino-acid sequence of the b-amylase from C. ... built for the b-amylasefrom C. sepium using the X-ray coordinates of the soybeanb-amylase (Fig. 4). According to the Ramachandran plot of this model the f and c angles of most of the residues ... by the inhibitors of the enzymatic activity.Therefore the iodine staining method is well suited forextensive kinetic analyses of purified b-amylases.Stability tests The heat stability of the...
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Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx

Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx

... confirmed by the kinetic analysis of a purified GPI-anchorless solubleversion of the mutant protein. The Kcat⁄ Kmvalue of the mutant TNSALP was less than one-tenth of the Kcat⁄ Kmvalue of the ... TNSALP (R433C). The rate of the intracellulartransport of the mutant protein was similar to that of the wild-type protein, as assessed by the acquisition of Endo H resistance. Besides, the mutant ... anti-TNSALP serum. The arrowhead indicates the top of the resolving gel. The values are the means of twoindependent experiments. (D) COS-1 cells expressing the wild-type TNSALP, or the TNSALP (V406A)...
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Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx

Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx

... than the value of 0.79 expected from theoretical consider-ations at a field strength of 11.7 T [37]. These results(together with those of the R2described above) suggesta high flexibility of the ... [31]. The residues with low NOE values (< 0.65) inCACW40A were Ile9 (at the C-cap of the 310-helix);Tyr20 (at the beginning of the first helix); Lys26 andAla30 (at the C-cap of the first ... 0where the coefficients of the cubic equation, a and b,are obtained from the coefficients of the linear regres-sion of the experimental J(xN) (i.e. the spectral densityfunction at the Larmor...
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

... of Cyn d 24. (A) Nucleotide and deduced amino acid sequences of Cyn d 24. The numberson the right of the figure indicate the positions of the nucleotide sequence. The numbers on the left of the ... [32,33].Formation of this type of oligosaccharide in BG60 hasbeen suggested to be the result of degradative reac-tions, rather than imperfect biosynthesis [13]. Of the plant allergens listed in the of cial ... 230proteins and about 65 of these are IgE binding pro-teins [20]; however, some of these are known to beeither isoforms or the degraded products of allergens.Furthermore, allergic patients...
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Báo cáo khóa học: Structural properties of the protein SV-IV potx

Báo cáo khóa học: Structural properties of the protein SV-IV potx

... identified by the massincrease of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species. From the data ... a-helix in the whole protein, and the 1–70region is poorly structured.SDS increases the a-helix content of proteins revealing the helical potential. The a-helix content of the monomericform of SV-IV ... measured by electrosprayMS. Theoretical mass is the mass calculated on the basis of the protein amino-acid sequence. The relative abundance refers to the ratio of the acetylated/unacetylated...
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Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

... the theoretical mass of the human PKIb (8468.2 Da) [11]. The identification of the gene product of human PKIb excludedit from the interference of human PKIb-70, which wastranslated from another ... mMsodium acetate buffer. The pooledfraction was stored at )20 °C for further study. The concentration of the proteins was determined by the method of Lowry et al. [14] and the purity was examinedby ... SDS/PAGE [15].Assay of human PKIb activity The activity of purified human PKIb was assayed by the inhibition of the catalytic subunit of cAPK in a 50 lLreaction containing 0.5 units of purified catalytic...
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Báo cáo khoa học: Cellular refractoriness to the heat-stable enterotoxin peptide is associated with alterations in levels of the differentially glycosylated forms of guanylyl cyclase C pdf

Báo cáo khoa học: Cellular refractoriness to the heat-stable enterotoxin peptide is associated with alterations in levels of the differentially glycosylated forms of guanylyl cyclase C pdf

... thoughtthey were able to bind the ST peptide (Fig. 2). Therefore,there appeared to be a correlation between the presence of the 145 kDa form of GC-C, which represents the matureglycosylated form of ... representing the amount of GC-C detected byST binding (Fig. 2C). Therefore, the fraction of GC-Cpresent in the membranes of desensitized cells possessedguanylyl cyclase activity, but this form of the ... onremoval of the ST peptide, which was correlated with areappearance of the 145 kDa form on the cell surface, fol-lowing processing of the endoplasmic reticulum-associatedpool of the 130 kDa...
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