Báo cáo Y học: Inhibition of SERCA Ca2+ pumps by 2-aminoethoxydiphenyl borate (2-APB) 2-APB reduces both Ca2+ binding and phosphoryl transfer from ATP, by interfering with the pathway leading to the Ca2+-binding sites ppt
... inhibition of SERCA (Eur. J. Biochem. 269) 3687
Inhibition of SERCA Ca
2+
pumps by 2-aminoethoxydiphenyl borate
(2-APB)
2-APB reduces both Ca
2+
binding and phosphoryl transfer from ATP, by interfering
with ... data to show that 2-APB can inhibit the
SERCA Ca
2+
pumps by reducing both the affinity of Ca
2+
binding and phosph...
... that the nucleotide -binding
domain is highly mobile in the presence of Ca
2þ
and can
come into close contact with the phosphorylation site by
simple thermal fluctuation [15].
From this type of analysis ... the
absence and presence of curcumin, and controls to estimate
nonspecific binding of ATP to the filter were performed as
described previously [16].
Phosp...
... hypo-
thesis is further confirmed by the observation that, differ-
ently from the effect of serum, the stimulatory effect of
PMA on ERK1/2 activity could also be totally abolished by
NAMI-A. Within ... to
the phosphorylation of ERK1/2 isoenzymes (Fig. 2B).
To verify the involvement of a PKC-dependent pathway
in both serum- and PMA-generated ERK1/2 phosphoryla-...
... 2). The sixfold stron ger
splicing inhibition by Cl-tetracycline may be explained by
the a dditional s ize and hydrophobicity introduced by the
substitution, as is often the case in ribozyme inhibition ... aminoglycoside,
kanamycin, the a minocyclitol glycoside, streptomycin, t he
penicillins, ampicillin and penicillin G, the aminocyclitol,
spectinomycin, tetracyclin...
... contribute to their pathogenicity by allowing
them to escape from the immune systems of their hosts.
The HA synthesized by Streptococci is not chemically
or structurally distinguishable from that synthesized ... mechanism of inhibition of HA synthesis by MU is
probably due to an alteration of the lipid environment of the
Streptococcal membranes.
MATERIALS...
... understanding of
the biosynthesis pathway and function of GPtdIns came
from the use of specific inhibitors of the GPtdIns
biosynthesis. A recently established fungi metabolite
(YW3548) was shown to ... 2-deoxy-2-fluoro-
D-glucose and 2-amino-2-deoxy-D-mannose) have been
used to study glycosylphosphatidylinositol (GPtdIns)
biosynthesis and GPtdIns protein anchoring in...
... night and the reaction’s completeness was
checked by thin layer chromatography. The reaction prod-
uct was inspissated by the rotary evaporator and consecu-
tively und covered with ether. The ...
Synthesis of the PNA building block backbone
In the next step, the synthesis of the PNA back-
bone monomer using the fluorenylmethoxycarbonyl
(Fmoc) protection occ...
... studies.
ACKNOWLEDGEMENTS
This study was supported by the PBTS Research Program with
financial aid from the Ministry of Economic Affairs and by the Integral
Structure Plan for the Northern Netherlands from the Dutch Develop-
ment ... composition of the EPS produced by
S. thermophilus 8S, the genetics and biochemistry of the
EPS biosynthesis as well as the...
... used instead of
the substrate to avoid decarbamoylation due to transcarb-
amoylation by the choline, generated from the substrate
hydrolyzed by the noncarbamoylated fraction of the
enzyme. Figure ... simultaneously to
the free and the acetylated enzyme as shown by structural
studies [30] and (b) the occupation of the anionic subsite at
the bottom o...
... also retains the
cytotoxicity, supporting the hypothesis that the cyto-
toxicity primarily arises from the reaction adduct or
the a-Syn oligomers rather than from free DA or the
a-Syn fibrils. Although ... reactions of a-Syn with quinone and DA. (A) Overlay of the
1
H-
15
N HSQC spectra of a-Syn (black) and
the reaction product of a-Syn and Q (red). (B) Over...