... ATP (25 mm ), and the other with ATP (0.1–20 mm) and fixed l-lysine (0.5 mm).For each assay, the LysU reaction mixture with addedl-lysine and ATP was transferred to the cuvette and equilibrated ... (1995) The crystal struc-ture of the lysyl-tRNA synthetase (LysU) from Escheri-chia coli. Structure 3, 163–176.3 Ito K, Oshima T, Mizuno T & Nakamura Y (1994)Regulation of lysyl-tRNA synthetase ... spectroscopy and thin-layer chromatography.Eur J Biochem 136, 469–479.18 Hughes SJ, Tanner JA, Hindley AD, Miller AD & GouldIR (2003) Functional asymmetry in the lysyl-tRNAsynthetase explored by...