Báo cáo Y học: Purification and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identification as a product of liver origin doc
Báo cáo Y học: Diffusion through channel derivatives of the Escherichia coli FhuA transport protein doc
... derivatives as indicated in the table. The molar masses and the absolute amounts of the inhibitors and the maltodextrinsadded to the filter paper discs are indicated. The size of the zones of growth ... to about half the area of the total cross-section [6].Removal of the globular domain may increase the flexibility of loop 4 and other surface exposed loops so that rapidlymoving loops may cause ... thatbinding of vitamin B12to BtuB alters the conformation and the dynamics of the TonB box segment [14].At the time when the crystal structure of FhuA was notyet available we arrived at an early tentative...
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Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
... they are also known as the E-NTPDases.Ecto-ATP-diphosphohydrolase (ecto-ATPDases) and ecto-ATPasesare two different subfamilies of the E-ATPases. E-type ATPases areATPases that have similar ... Lewis-Carl,S.&Kirley,T.L.(1997)Immunolocalizationoftheecto-ATPase and ecto-apyrase (ATPDase) in chicken gizzard and stomach: purification and N-terminal sequence of the stomachecto-apyrase. ... CaATPase activity of the purifiedchicken liver ecto-ATPDase is 30% of the MgATPaseactivity at a divalent ion-ATP concentration of 5 mMatpH 7.4. On the other hand, the CaADPase activity is...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... soybean b-amylase (Cys82, Cys97, Cys208 and Cys343).On the analogy of the soybean b-amylase, the activesite of the C. sepium b-amylase most probably consists of a cleft located between the barrel ... against b-amylase and C. sepiumRNase-related protein (CalsepRRP)Polyclonal antibodies were raised against b-amylase and the unglycosylated isoform of CalsepRRP [14]. Male NewZealand white rabbits ... maltose and cyclohexaamylose b-amylase activity was measuredusing the iodine staining method with soluble starch as a substrate [21]. The inhibition type of glucose, maltose and cyclohexaamylose...
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Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx
... neuroprotection;quinolinic acid; tryptophan metabolism.Rapid progress in the pathophysiology of human diseaseshas always been hampered by the availability of humantissue, aesthetics, and ethical considerations. The ... strong anionic and cationic sepa-rations. The purity of the enzyme was determined by SDS/PAGE, and the molecular mass verified by MALDI-TOFMS. The monomeric molecular mass of 52.4 kDa deter-mined ... with the Bradford assay [13]. Recombinant humankynureninase from the final anion-exchange step wasassayed for purity using both SDS/PAGE and MALDI-TOF MS of the whole protein. Both purified and...
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Báo cáo Y học: Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod pdf
... assayAfter each chromatographic step the inhibitory activity of collected fractions was determined using papain as the enzyme in the assay [6]. The same assay was used todetermine inhibitory activites ... kininogen carries biantennary and triantennary N-glycans that are terminated by a2 ,3-linkedsialic acids, a high number of which are O-acetylated. About1/3 of the glycans carry sulfate at N-acetylglucosamine ... Biosystems,Framingham MA, USA) tip, and a sample of the flow-through carrying the glycans was drop-dialysed and analysed by MALDI-TOF MS.RESULTSIsolation of cysteine proteinase inhibitors The first...
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Báo cáo Y học: Purification and characterization of three galactose specific lectins from Mulberry seeds (Morus sp.) ppt
... buffercontaining 0.2M galactose.Protein and carbohydrate analysis The concentration of protein was measured by the method of Lowry et al. [13] using BSA as the standard. The presence of sugar in the ... Purification and characterization of three galactose specific lectinsfrom Mulberry seeds (Morussp.)Tanzima Yeasmin, Md Abul Kashem Tang, Abdur Razzaque and Nurul AbsarDepartment of Biochemistry, ... Although the yield of these proteinswas found to be decreased by the purification steps and over96% of protein was lost, the purification of the proteins wasincreased after each subsequent purification...
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Báo cáo Y học: Purification and characterization of VanXYC, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174 docx
... polymerase(Stratagene) was used to amplify vanXYCusingpAT70 4as template with primers A (5¢-GCTAGGTCTCAATGAACACATTACAATT-3¢)andB(5¢-TATGGAATTCTCATGCGAACTGCCTCA-3¢) that included BsaIandEcoRIrestriction ... bi[tris(hydroxymethyl)-meth-ylamino] propane and 10 lL10mMD-Ala-D-Ala, incuba-ted at 37 °C for 30 min, and assayed forD,D-dipeptidaseactivity using the D-amino acid oxidase assay.Kinetic analysis of VanXYCHis6–VanXYC(2.25 ... Purification of VanXYC.Lane1,molecular mass standards (in kDa); Lane 2, partially pure VanXYCafter gel filtration chromatography. (B) Purification of MBP-VanXYC.Lane 1, molecular mass standards;...
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Báo cáo Y học: Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides pot
... enzyme required the adenosylcobalamin coenzyme for catalyticactivity and the Kmfor the cofactor was 8 lM. Inactivation of the enzyme was observed by both glycerol and cyano-cobalamin. The ... °C.Dehydratase assaysTwo methods were used for the determination of the activity. During the purification and characterization enzyme steps, the assay was carried out using the 3-methyl-2-benzothiazolinone ... showed that the main band wasreleased into three subunits migrating at the same positions as the large, medium and small subunits of the dioldehydratase. However, the band of 59 kDa was not alignedwith...
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... SE of n ¼ 3 separate determinations.Table 2. Effect of various divalent metal cations and EDTA on the activity of SAP1 and SAP2. The standard assay A was used except that the phosphoenolpyruvate ... the hydrolysis of 1 lmol of substrate per min at 25 °C.Phosphatase assay A For routine measurements of AP activity, the hydrolysis of phosphoenolpyruvate to pyruvate was coupled to the lactatedehydrogenase ... the filtrate concentrated as described below.AP assaysAll assays were linear with respect to time and concentration of enzyme assayed. One unit (U) of activity is defined as the amount of AP resulting...
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Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
... °C.Synthesis of XAC-agarose gel The ligand af®nity gel was prepared based on the methoddescribed by Nakata [15]. XAC was dissolved in dimethyl-sulfoxide at a con centration of 0 .5 mgámL)1.Af®-Gel10resin ... N-terminal proteinsequencer (Procise 494, Applied Biosystems).Protein assay and amino-acid analysisProtein concentrations were determined by the Amido blackassay [24] using BSA as a standard. ... column was slow even a tincreased temperature (25 °C) resulting i n a dilute eluate. The low af®nity antagonist theophylline was used forelution as high af®nity hA2aR antagonists are hardlysoluble...
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