... Shigeyuki Imamura1and Masatake Ohnishi21Department Diagnostics Research and Development, Division of Fine Chemicals and Diagnostics, Asahi Kasei Pharma Corporation,Shizuoka, Japan;2Department ... testosteroni 3a- HSD/CR and rat liver 3a- HSD.Materials and methodsMaterialsNAD+,NADP+, NAAD+, NADH, and NADPH (Ori-ental Yeast Co., Ltd in Japan), steroids (Sigma Chem. Co.)and other chemicals ... [11–14]. Although structurally different, both HSDs arefunctionally similar; the AKRs are monomeric and have an a/ b-barrel fold, whereas the SDRs are dimeric or tetramericand contain a Rossmann...